| Co-Investigator(Kenkyū-buntansha) |
小守 壽文 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00252677)
兒玉 幸修 長崎大学, 病院(医学系), 准教授 (50448510)
住田 吉慶 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
佐々木 均 長崎大学, 病院(医学系), 教授 (00170689)
鎌倉 慎治 東北大学, 医工学研究科, 教授 (80224640)
三浦 桂一郎 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10634446)
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| Budget Amount *help |
¥42,250,000 (Direct Cost: ¥32,500,000、Indirect Cost: ¥9,750,000)
Fiscal Year 2021: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2020: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2019: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2018: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Outline of Final Research Achievements |
This study investigated whether a gene-activated matrix (GAM) composed of nanoballs containing pDNAs encoding BMP4 (pBMP4) could promote bone augmentation. We prepared nanoballs (BMP4-nanoballs) constructed with pBMP4 and dendrigraft poly-L-lysine (a cationic polymer) coated by γ-polyglutamic acid (an anionic polymer) and determined their biological functions in vitro and in vivo. Next, GAMs were manufactured by mixing nanoballs with 2% atelocollagen and β-TCP granules and lyophilizing for bone augmentation. GAMs were then transplanted to rat cranial bone surfaces under periosteum. As results, bone augmentation was clearly recognized for up to 8 weeks in transplanted GAMs containing BMP4-nanoballs. Notably, only 0.001mg of BMP4-nanoballs induced a sufficient volume of new bone, while 1mg of naked pDNAs were required to induce the same level of bone augmentation. These data suggest that applying this anionic vector to appropriate matrices can facilitate GAM-based bone engineering.
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