Molecular epidemiology study of the colistin-resistant bacteria dissemination in Vietnam

• Project/Area Number: 17H01687
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Laboratory medicine
• Research Institution: Gifu University (2019-2020); Osaka University (2017-2018)
• Principal Investigator: Yamamoto Yoshimasa 岐阜大学, 高等研究院, 招へい教員 (20010100)
• Co-Investigator(Kenkyū-buntansha): 河原 隆二 地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 主任研究員 (10332454) ; 田中 香お里 岐阜大学, 高等研究院, 教授 (20242729) ; 朝野 和典 大阪大学, 医学系研究科, 教授 (40202204) ; 中村 昇太 大阪大学, 微生物病研究所, 特任准教授(常勤) (90432434)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000); Fiscal Year 2019: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2018: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000); Fiscal Year 2017: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
• Keywords: 薬剤耐性菌 / コミュニテイ / コリスチン耐性 / 伝達性耐性遺伝子 / 薬剤耐性 / コリスチン / ベトナム / 住民 / 細菌

Outline of Final Research Achievements

The study was conducted with the aim of elucidating the actual condition of colistin-resistant bacteria dissemination in Vietnam from a molecular epidemiological point of view and obtaining basic data that are necessary for making public health measures to further expansion of colistin-resistant bacteria.
It was clarified by the study that 70% of the community residents in the target area were infected with colistin-resistant Escherichia coli (COR-EC) possessing the mobile resistance gene mcr. In addition, all most all domestic livestock (95%) in the community were contaminated with mcr-positive COR-EC. From these results, it was obvious that the epidemic in the community of the target area was serious. As a result of genome analysis of the colistin-resistant isolates, 36% of the isolates had the mobile mcr incorporated on the chromosome, and the mcr transposon structure analysis showed that the dissemination of colistin-resistant bacteria was being stabilized in the community.

Academic Significance and Societal Importance of the Research Achievements

本研究でベトナムの地方農村コミュニテイでコリスチン耐性菌の蔓延が住民ならびに飼育家畜で深刻な状況にあることが判明した。加えてこのような状況が一時的では無く慢性化する可能性が分離菌のゲノム解析から明らかとなった。同様な状況は比較研究として行ったエクアドルの地方コミュニテイでも認められた事から、程度の差は有るが途上国に共通する事象と考えられ、早急な耐性菌蔓延対策が求められる。

Research Products

• [Int'l Joint Research] タイビン医科薬科大学(ベトナム)
• [Int'l Joint Research] Universidad De Las Americas(エクアドル)
• [Int'l Joint Research] タイビン医科薬科大学(ベトナム)
• [Int'l Joint Research] アメリカ大学（UDLA)(エクアドル)
• [Int'l Joint Research] 南フロリダ大学(米国)
• [Int'l Joint Research] タイビン医科薬科大学(ベトナム)
• [Journal Article] High Prevalence of Colistin-Resistant Escherichia coli with Chromosomally Carried mcr-1 in Healthy Residents in Vietnam (2020)
  • Author(s): Yamaguchi Takahiro、Kawahara Ryuji、Hamamoto Kouta、Hirai Itaru、Khong Diep Thi、Nguyen Thang Nam、Tran Hoa Thi、Motooka Daisuke、Nakamura Shota、Yamamoto Yoshimasa
  • Journal Title: mSphere Volume: 5 Issue: 2
  • DOI: 10.1128/msphere.00117-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Whole-genome sequencing and comparative analysis of the genomes of Bacteroides thetaiotaomicron and Escherichia coli isolated from a healthy resident in Vietnam (2020)
  • Author(s): Mohsin Mashkoor、Tanaka Kaori、Kawahara Ryuji、Kondo Shinji、Noguchi Hideki、Motooka Daisuke、Nakamura Shota、Khong Diep Thi、Nguyen Thang Nam、Hoang Trong Nang、Yamamoto Yoshimasa
  • Journal Title: Journal of Global Antimicrobial Resistance Volume: 21 Pages: 65-67
  • DOI: 10.1016/j.jgar.2020.02.034
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantification and long-term carriage study of human extended-spectrum/AmpC β-lactamase-producing Escherichia coli after international travel to Vietnam (2020)
  • Author(s): Tatsuya Nakayamaa, Yuko Kumedac, Ryuji Kawaharad, Yoshimasa Yamamoto
  • Journal Title: Journal of Global Antimicrobial Resistance Volume: 21 Pages: 229-234
  • DOI: 10.1016/j.jgar.2019.11.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] <p>Prevalence Of <em>mcr-1</em> Among Cefotaxime-Resistant Commensal <em>Escherichia coli</em> In Residents Of Vietnam</p> (2019)
  • Author(s): Kawahara Ryuji、Khong Diep Thi、Le Ha Viet、Phan Quang Ngoc、Nguyen Thang Nam、Yamaguchi Takahiro、Kumeda Yuko、Yamamoto Yoshimasa
  • Journal Title: Infection and Drug Resistance Volume: Volume 12 Pages: 3317-3325
  • DOI: 10.2147/idr.s224545
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Most Domestic Livestock Possess Colistin-Resistant Commensal Escherichia coli Harboring mcr in a Rural Community in Vietnam (2019)
  • Author(s): Kawahara Ryuji、Fujiya Yoshihiro、Yamaguchi Takahiro、Khong Diep Thi、Nguyen Thang Nam、Tran Hoa Thi、Yamamoto Yoshimasa
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 63 Issue: 6 Pages: 1-2
  • DOI: 10.1128/aac.00594-19
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Wide dissemination of colistin-resistant Escherichia coli with the mobile resistance gene mcr in healthy residents in Vietnam (2019)
  • Author(s): Yamamoto, Y.,Kawahara, R., Fujiya, Y., Sasaki, T., Hirai, I., Khong, D. T., Nguyen, T. N., Nguyen, B. X.
  • Journal Title: J Antimicrob Chemother Volume: 74 Issue: 2 Pages: 523-524
  • DOI: 10.1093/jac/dky435
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador (2019)
  • Author(s): Yamamoto, Y., Calvopina, M., Izurieta, R., Villacres, I., Kawahara, R., Sasaki, M., Yamamoto, M.
  • Journal Title: BMC Res Notes Volume: 12 Issue: 1 Pages: 121-121
  • DOI: 10.1186/s13104-019-4144-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Carriage of colistin-resistant, extended-spectrum beta-lactamase-producing Escherichia coli harboring the mcr-1 resistance gene after short-term international travel to Vietnam (2018)
  • Author(s): Nakayama, T., Kumeda, Y., Kawahara, R., Yamaguchi, T.,Yamamoto, Y.
  • Journal Title: Infect Drug Resist Volume: 11 Pages: 391-395
  • DOI: 10.2147/idr.s153178
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli and residual antimicrobials in the environment in Vietnam (2018)
  • Author(s): Yamasaki, S., Le, T. D., Vien, M. Q., Van Dang, C., Yamamoto, Y.
  • Journal Title: Anim Health Res Rev Volume: 18 Issue: 2 Pages: 128-135
  • DOI: 10.1017/s1466252317000160
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Presence of Colistin resistance gene mcr-1 and -3 in ESBL producing Escherichia coli isolated from food in Ho Chi Minh City, Vietnam (2018)
  • Author(s): Yamaguchi T., Kawahara R., Harada K., Teruya S., Nakayama T., Motooka D., Nakamura S., Nguyen D P., Kumeda Y.,Dang V C.,Hirata K.,Yamamoto Y.
  • Journal Title: FEMS Microbiology Letters Volume: 印刷中 Issue: 11 Pages: 1-8
  • DOI: 10.1093/femsle/fny100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ベトナムにおける料理を汚染している基質特異性拡張型ベータラクタマーゼ産生菌及びヒト腸内細菌叢への影響に関する研究 (2020)
  • Author(s): 中山達哉、久米田裕子、河原隆二、山本容正
  • Organizer: 日本食品衛生学会
• [Presentation] ベトナムの豚肉から分離したmcr-3を有するコリスチン耐性大腸菌の解析 (2018)
  • Author(s): Yamaguchi, T., et al.
  • Organizer: 第91回日本細菌学会総会
• [Presentation] Extremely High Prevalence of Colistin-Resistant Escherichia coli with mcr in Healthy Residents in Vietnam (2018)
  • Author(s): Y. Yamamoto, R. Kawahara, Y. Fujiya, T. Sasaki, I. Hirai, D. T. Khong, T. N. Nguyen
  • Organizer: ASM Microb 2018