| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Outline of Final Research Achievements |
Local perturbations of focused laser beam actively manipulate functional molecules on neurons, and transient intracellular molecular movements in response to the perturbations were revealed by fluorescence analysis. Cell surface receptor molecules were optically trapped as laser intensity or days in culture increased, indicating that optical trapping dynamics were dependent on the initial assembly state. Furthermore, simultaneous measurement of intracellular electrical activity and microscopic fluorescence analysis revealed that synaptic transmission strength in neurons may increase based on optical trapping of receptor molecules.
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