| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
Homology-directed repair (HDR) is critical for the repair of DNA double-strand breaks, while HDR between similar but non-identical sequences can give rise to chromosome instability. The quality of HDR is maintained by the mismatch repair (MMR) pathway. However, the molecular mechanism of these reactions remains largely uncertain. In this study, we recapitulated an HDR reaction in Xenopus egg extracts, identified factors that maintain the accuracy of HDR, and studied the quality-control mechanism of HDR. We also discovered a novel mechanism that assists in the MMR reaction on chromatin. These studies contribute to the understanding of the MMR-dependent quality-control system for HDR and DNA replication.
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