| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
In order to evaluate the biological effects of chemicals, it is important to develop methods using living organisms in addition to the conventional analytical approaches. Chemicals have a very wide range of mod of actions, one of which is hormone-like chemicals. In this study, we aimed to introduce a human-type chemical response system based on nuclear receptors into Daphnia magna. We improved the genome editing efficiency by modifying the established transgene transfer system, and established a system that efficiently transfers foreign genes without position effects. Using this system, we succeeded to introduce a human-type chemical response system. We confirmed the gene response of this human-type transgenic Daphnia magna by actual exposure of hormone like chemicals.
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