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Elucidation of N2O production/consumption mechanisms in biofilms and development of a wastewater treatment technology mitigating N2O emission

Research Project

Project/Area Number 17H01893
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Terada Akihiko  東京農工大学, 工学(系)研究科(研究院), 教授 (30434327)

Co-Investigator(Kenkyū-buntansha) 藤原 拓  高知大学, 教育研究部自然科学系農学部門, 教授 (10314981)
堀 知行  国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (20509533)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords亜酸化窒素 / 亜酸化窒素還元細菌 / ガス透過膜 / バイオフィルム / 15Nトレーサー法 / 微生物叢解析 / 硝化 / 脱窒 / 生成機構解明 / N2O還元細菌 / 対抗拡散方式 / 亜酸化窒素消費細菌 / pH / 硫化水素 / 対抗拡散型バイオフィルム / 硝化・脱窒 / 微小電極 / 生成機構 / 排水処理 / 細菌
Outline of Final Research Achievements

This study aimed at developing a novel biofilm process mitigating the emissions of highly potent greenhouse gas, nitrous oxide (N2O). The process is based on a biofilm on a gas-permeable membrane where the exhaust gas from an aeration tank, containing N2O, is supplied. The reactor operation demonstrated that the biofilm process substantially alleviates N2O emission by bacteria consuming N2O in the biofilm. The microbial community analysis successfully identified promising N2O-consuming bacteria. Furthermore, physiological experiments revealed favorable ranges of temperature and oxygen levels for the selected bacteria to maximize their N2O consumption potentials.

Academic Significance and Societal Importance of the Research Achievements

亜酸化窒素(N2O)は第三の温室効果ガスと言われ、排水の高度処理を行っている施設での排出削減が求められている。N2Oは高度処理施設において、排水中に含まれる窒素化合物を窒素ガスに変換する際に副生成物として排出される。本研究は、このN2Oを効率的に削減できる新しい技術を提案している。本研究で得られた成果は、提案した技術の排水処理施設への導入によるN2O排出量削減につなげられる。また、N2Oを効率的に窒素ガスに分解できる細菌群を同定したため、農地などへの適用により、N2O排出の削減が期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (30 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (17 results) (of which Int'l Joint Research: 9 results,  Invited: 9 results)

  • [Int'l Joint Research] ダルムシュタット工科大学(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] デンマーク工科大学(デンマーク)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] デンマーク工科大学(デンマーク)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ダルムシュタット工科大学(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] デンマーク工科大学(デンマーク)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ノートルダム大学(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Exploration and enrichment of methane-oxidizing bacteria derived from a rice paddy field emitting highly concentrated methane2020

    • Author(s)
      Yasuda, S., Toyoda, R., Agrawal, S., Suenaga, T., Riya, S., Hori, T., Lackner, S., Hosomi, M., Terada, A.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: in press Issue: 3 Pages: 311-318

    • DOI

      10.1016/j.jbiosc.2020.04.006

    • NAID

      40022351710

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Startup, performance, and microbial communities of an anammox reactor inoculated with indigenous sludge for the treatment of high-salinity and mesophilic underground brine2019

    • Author(s)
      Yokota, N., Mineshima, R., Yamaguchi, H., Hirase, T., Ishikawa, H., Azuma, T., Hosomi, M., Terada, A.
    • Journal Title

      Clean Technologies and Environmental Policy

      Volume: 21 Issue: 5 Pages: 1001-1011

    • DOI

      10.1007/s10098-019-01688-y

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enrichment, Isolation, and Characterization of High-Affinity N2O-Reducing Bacteria in a Gas-Permeable Membrane Reactor2019

    • Author(s)
      Suenaga, T., Hori, T., Riya, S., Hosomi, M., Smets, Barth F., Terada, A.
    • Journal Title

      Environmental Science & Technology

      Volume: 53 Issue: 20 Pages: 12101-12112

    • DOI

      10.1021/acs.est.9b02237

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Determining uncertainties in PICRUSt analysis - An easy approach for autotrophic nitrogen removal2019

    • Author(s)
      Agrawal, S., Kinh, C. T., Schwartz, T., Hosomi, M., Terada, A., Lackner, S.
    • Journal Title

      Biochemical Engineering Journal

      Volume: 152 Pages: 107328-107328

    • DOI

      10.1016/j.bej.2019.107328

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Immobilization of Azospira sp. strain I13 by gel entrapment for mitigation of N 2 O from biological wastewater treatment plants: Biokinetic characterization and modeling2018

    • Author(s)
      Suenaga Toshikazu、Aoyagi Ryo、Sakamoto Nozomi、Riya Shohei、Ohashi Hidenori、Hosomi Masaaki、Tokuyama Hideaki、Terada Akihiko
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中 Issue: 2 Pages: 213-219

    • DOI

      10.1016/j.jbiosc.2018.02.014

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Draft genome sequence of Azospira sp. strain I13, a nitrous oxide-reducing bacterium harboring clade II type nosZ2018

    • Author(s)
      Toshikazu Suenaga、Tomo Aoyagi、Masaaki Hosomi、Tomoyuki Hori、Akihiko Terada
    • Journal Title

      Genome Announcements

      Volume: Accepted

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of hotspots for NO and N 2 O production and consumption in counter- and co-diffusion biofilms for simultaneous nitrification and denitrification2017

    • Author(s)
      Kinh Co Thi、Riya Shohei、Hosomi Masaaki、Terada Akihiko
    • Journal Title

      Bioresource Technology

      Volume: 245 Pages: 318-324

    • DOI

      10.1016/j.biortech.2017.08.051

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 15Nトレーサー法を用いた脱窒細菌のN2O還元ポテンシャルの評価2020

    • Author(s)
      大場康平、末永俊和、利谷翔平、寺田昭彦
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A membrane-aerated biofilm reactor for cost-effective nitrogen removal with low greenhouse gas emissions: Insights from macro and micro scale observations2019

    • Author(s)
      Akihiko Terada
    • Organizer
      The 5th EnvironmentAsia International Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ガス透過膜を用いたバイオフィルムリアクターの省エネ化・高機能化に向けた現状と課題2019

    • Author(s)
      寺田昭彦
    • Organizer
      第36回ニューメンブレンテクノロジーシンポジウム2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Activity, abundance, and identification of N2O-reducing bacteria present in Anammox biomass - Combination of 15N tracer and molecular analyses2019

    • Author(s)
      Suenaga, T., Ota, T., Hori, T., Riya, S., Hosomi, M., Chandran, K., Lackner, S., Smets, B.F., Terada, A.
    • Organizer
      The 8th IWA Microbial Ecology and Water Engineering Specialist Conference (MEWE2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Counter-current substrate diffusion biofilms for nitrogen removal from wastewaters2019

    • Author(s)
      Akihiko Terada
    • Organizer
      The 2nd International Conference on Non-point Source Pollution Control and Aquatic Ecosystem Protection (NPAE-2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A Membrane-aerated Biofilm Reactor for Effective Nitrogen Removal from Wastewaters: Opportunities and Challenges2019

    • Author(s)
      Akihiko Terada
    • Organizer
      IEEC & BWR 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 下水汚泥処理工程における亜酸化窒素排出メカニズムの検討2019

    • Author(s)
      藤川真理子,野村洋平,末永俊和,寺田昭彦,藤原 拓
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アナモックス汚泥に棲息するN2O還元細菌のポテンシャル評価2019

    • Author(s)
      太田琢,末永俊和,細見正明,寺田昭彦
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 排水中の微量元素が部分硝化法におけるN2O生成に及ぼす影響2019

    • Author(s)
      大野哲,末永俊和,細見正明,寺田昭彦
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 工学システムの進展から見る窒素マネジメントの現状と課題2018

    • Author(s)
      寺田昭彦
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 対抗拡散型バイオフィルムによる水処理技術:窒素除去の観点から見た利点と課題2018

    • Author(s)
      寺田昭彦
    • Organizer
      化学工学会第50回秋季大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] A membrane-aerated biofilm reactor for efficient single-stage nitrogen removal and mitigation of nitrous oxide emission: Proof-of-concept from biofilm depth profile analysis2018

    • Author(s)
      Terada, A., Kinh, C.T., Suenaga, T., Hori, T., Riya, S., Hosomi, M.
    • Organizer
      IWA Water Reuse 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] バイオフィルムに関する最新の研究動向:形成抑制から利用技術まで2017

    • Author(s)
      寺田昭彦
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Nitrous oxide emission from cost-effective nitrogen removal systems: Mechanisms and mitigation strategies2017

    • Author(s)
      Akihiko Terada
    • Organizer
      2017 International Conference on Environmental Pollution Control
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Counter-current substrate diffusion biofilm as an innovative strategy for effective nitrogen removal from wastewaters: Principles, performances and in situ biofilm analyses2017

    • Author(s)
      Akihiko Terada
    • Organizer
      The 8th China-Japan Symposium on Chemical Engineering
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effect of nitrate and hydrogen sulfide on N2O emission in sewage sludge treatment system2017

    • Author(s)
      Qian Yang、Sijing Jia、Shaolan Ding、Taku Fujiwara
    • Organizer
      The 10th CESE Conference International Conference in Challenges in Environmental Science & Engineering
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Membrane-aerated biofilm reactors for nitrogen removal and mitigation of nitrous oxide emission from wastewater streams2017

    • Author(s)
      Akihiko Terada
    • Organizer
      EnvironmentAsia 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2017-04-28   Modified: 2022-05-20  

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