| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
This study aimed to elucidate the formation and maintenance mechanism of solid-phase humic substance (humin, humic substances insoluble in any pH), functioning as energy source for the reducing reactions of anaerobic microorganisms, important reactions for environmental remediation. Carried out were the chemical structural analysis of redox center in humin, its electrochemical characterization, and the analysis of community structure of anaerobic microbial consortium reducing humin. The comparative study of the various spectra for humins with different extracellular electron mediating efficiencies suggested the association of peptide glycan structure in the extracellular electron mediating function. Electrochemical analysis using electrochemical impedance spectra showed the trend agreeing with the microbial metabolism but not with the values. Microbial consortium containing the reaction of humin reduction was composed of mostly Clostridiales (>90%).
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