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Functional typing of reductive dehalogenase in dehalorespiring bacteria

Research Project

Project/Area Number 17H01900
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Modeling and technologies for environmental conservation and remediation
Research InstitutionNagoya Institute of Technology

Principal Investigator

Yoshida Naoko  名古屋工業大学, 工学(系)研究科(研究院), 准教授 (10432220)

Co-Investigator(Kenkyū-buntansha) 押木 守  長岡工業高等専門学校, 環境都市工学科, 准教授 (90540865)
野中 里佐  獨協医科大学, 医学部, 講師 (70363265)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2017: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsバイオレメディエーション / 還元的脱塩素化 / Dehalococcoides / 脱ハロゲン化呼吸細菌 / Geobacter / 脱ハロゲン化酵素 / Dehalobacter / 還元的脱ハロゲン化 / 脱塩素化 / 脱塩素化酵素
Outline of Final Research Achievements

This study newly isolated the D. mccarty NIT01, which is applicable for bio-augmentation of groundwater contaminated with chloroethene. The strain is capable of dechlorinating up to 4 mM TCE and has 19 dehalogenase (Rdh)-genes in the genome. The strain can be easily cultivated in large quantities and has high Rdh activity due to the involvement of multiple Rdhs, including known Rdh(Vcr) and unidentified Rdh. On the other hand, Geobacter sp. AY which has a prominent 1,2-DCA dechlorination rate among known DCA dechlorinators. The strain AY has a single copy of rdh in the genome, although the rdh (AY-dcaA) is constitutively transcribed 6.6 times that of rpoB. The AY-DcaA was expressed much, but the 1,2DCA dichlorination rate was relatively lower than that observed in known dechlorinators. Based on the above, the superior transcription and expression of rdh attributed the high dichlorination activity of 1,2-DCA by the strain AY.

Academic Significance and Societal Importance of the Research Achievements

学術的意義は、高い脱塩素化活性をもつ2つの脱ハロゲン化呼吸細菌,Dehalococcoidesmccartyi NIT01およびGeobacter sp. AYの活性の高さが、複数の脱塩素化酵素の関与、またはrdh遺伝子の恒常的・活発な転写に起因することを示した点にある.社会的意義は、簡便に培養できる微生物が大学の成果有体物として得られ、本株を利用したバイオオーグメンテーションがバイオレメディエーション指針の適合確認を得たことにある.本株は今後民間企業にひろく浄化に利用されることが期待できる.また、本研究で確立したrdhの抽出・精製技術は、地球化学的なハロゲンの循環把握に貢献できる

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (11 results)

All 2020 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Bacteroides sedimenti sp. nov., isolated from a chloroethenes-dechlorinating consortium enriched from river sediment2018

    • Author(s)
      Mohamed Ismaeil, Naoko Yoshida, Arata Katayama
    • Journal Title

      Journal of Microbiology

      Volume: 56 Issue: 9 Pages: 619-627

    • DOI

      10.1007/s12275-018-8187-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Multiple Dehalogenase Genes Involved in Tetrachloroethene-to-Ethene Dechlorination in a Dehalococcoides-Dominated Enrichment Culture2017

    • Author(s)
      Ismaeil Mohamed、Yoshida Naoko、Katayama Arata
    • Journal Title

      BioMed Research International

      Volume: 2017 Pages: 1-12

    • DOI

      10.1155/2017/9191086

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 塩素化エチレンを脱塩素化するDehalococcoides mccartyi NIT01株の大量培養および脱塩素化シミュレーション2020

    • Author(s)
      森田 悠揮 吉田奈央子
    • Organizer
      土木学会中部支部発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 塩素化エチレンを脱塩素化する Dehalococcoides mccartyi NIT01 株の大量培養手法の検討および分解シミュレーシ ョン2020

    • Author(s)
      森田 悠揮 吉田奈央子
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Dehalorespiring bacteria: from isolation to augmentation2019

    • Author(s)
      Naoko Yoshida
    • Organizer
      5th International Conference on Environmental Pollution and Health
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ミカエリス・メンテン式を用いた脱ハロゲン化呼吸細菌の培養シミュレーション2018

    • Author(s)
      日下部俊弥, 吉田奈央子
    • Organizer
      第24回 地下水・土壌汚染とその防止対策に関する研究集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 新規Dehalococcoides 属細菌の分離の試み2018

    • Author(s)
      吉田奈央子, Mohamed Ismael, 日下部俊弥, 片山新太
    • Organizer
      第24回 地下水・土壌汚染とその防止対策に関する研究集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Bacteroides sediment i sp. nov., a novel species of the genus Bacteroides which enhances the dechlorination of cis-chloroethene by Dehalococcoides2018

    • Author(s)
      M Ismaeil, N Yoshida, A Katayama
    • Organizer
      10th Asian Symposium on Microbial Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation and cultivation of dehalorespiring bacteria for bio-augmentation of aquifer contaminated with organohalides2018

    • Author(s)
      Naoko Yoshida
    • Organizer
      4th International Conference on Environmental Pollution and Health
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Discovery of an Anaerolineae bacerium capable of reductive dehalogenation of organohalogen compounds2018

    • Author(s)
      N Nakahara, E Tasumi, N Yoshida, M Nobu, H Tamaki, T Yamaguchi, K Takai, H Imachi
    • Organizer
      10th Asian Symposium on Microbial Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] トリクロロエチレン及び1,1,2-トリクロロエタンをエチレンに分解するデハロコッコイデス属細菌、浄化剤、及び浄化方法2019

    • Inventor(s)
      吉田奈央子
    • Industrial Property Rights Holder
      名古屋工業大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-115452
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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