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Microbial production of elastic polymers with ordered structure

Research Project

Project/Area Number 17H01902
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental conscious materials and recycle
Research InstitutionHokkaido University

Principal Investigator

Matsumoto Ken'ichiro  北海道大学, 工学研究院, 教授 (80360642)

Co-Investigator(Kenkyū-buntansha) 大井 俊彦  北海道大学, 工学研究院, 准教授 (40223713)
佐藤 敏文  北海道大学, 工学研究院, 教授 (80291235)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Keywords生分解性プラスチック / 生合成 / 自己組織化 / バイオベースプラスチック / 海洋分解性 / ブロック共重合体 / 微生物産生プラスチック / 生分解性材料 / バイオベースポリマー
Outline of Final Research Achievements

Bacterial polyester polyhydroxyalkanoates (PHAs) are biobased and biodegradable plastic, which are commercially used in Japan since 2019. To further expand the use of the material, the regulation of physical properties is an important research target. The conventional but effective strategy is random copolymerization, which increases the resistance to impact. We developed a novel method to control the monomer sequence of the polymer. In fact, the obtained sequence-regulated PHAs exhibited superior properties to the conventional polymers. These results indicated the usefulness of the sequence-regulating technique of PHA for creating the next-generation materials.

Academic Significance and Societal Importance of the Research Achievements

高分子が生合成されるという現象は、DNAやタンパク質などの合成に代表されるように生命の根源に関わる現象であり、その反応機構や制御機構には基礎科学的関心が持たれる。本研究成果の学術的意義は、生合成ポリマーの一つであるPHAに関して、これまでに知られていなかった生合成機構が存在することを示したことである。一方で社会的意義は、環境低負荷型材料として期待されるPHAの物性を改良するために、従来法とは異なる構造制御法を見出したことである。これにより、さらに多様な物性を持つPHAの生合成が可能になる可能性が拓けた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (13 results)

All 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] HES-SO(スイス)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Synergy of valine and threonine supplementation on poly(2-hydroxybutyrate-block-3-hydroxybutyrate) synthesis in engineered Escherichia coli expressing chimeric polyhydroxyalkanoate synthase2020

    • Author(s)
      Sudo Maho、Hori Chiaki、Ooi Toshihiko、Mizuno Shoji、Tsuge Takeharu、Matsumoto Ken'ichiro
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 129 Issue: 3 Pages: 302-306

    • DOI

      10.1016/j.jbiosc.2019.09.018

    • NAID

      120006980040

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ribulose-1,5-bisphosphate carboxylase/oxidase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli.2019

    • Author(s)
      Matsumoto K, Saito J, Yokoo T, Hori C, Nagata A, Kudoh Y, Ooi T, Taguchi S.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 印刷中 Issue: 3 Pages: 302-306

    • DOI

      10.1016/j.jbiosc.2019.03.002

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased Production and Molecular Weight of Artificial Polyhydroxyalkanoate Poly(2-hydroxybutyrate) Above the Glass Transition Temperature Threshold2019

    • Author(s)
      Matsumoto Ken'ichiro、Kageyama Yuki
    • Journal Title

      Frontiers in Bioengineering and Biotechnology

      Volume: 7 Pages: 177-177

    • DOI

      10.3389/fbioe.2019.00177

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In Vitro Analysis of d-Lactyl-CoA-Polymerizing Polyhydroxyalkanoate Synthase in Polylactate and Poly(lactate-co-3-hydroxybutyrate) Syntheses2018

    • Author(s)
      Ken’ichiro Matsumoto, Midori Iijima, Chiaki Hori, Camila Utsunomia, Toshihiko Ooi, and Seiichi Taguchi
    • Journal Title

      Biomacromolecules

      Volume: 19 Issue: 7 Pages: 28892895-28892895

    • DOI

      10.1021/acs.biomac.8b00454

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes of intracellular monomer levels regulate block sequence of polyhydroxyalkanoates in engineered Escherichia coli.2018

    • Author(s)
      Matsumoto K, Hori C, Fujii R, Takaya M, Ooba T, Ooi T, IsonoT, Satoh T, Taguchi S.
    • Journal Title

      Biomacromolecules

      Volume: 19 Issue: 2 Pages: 662-671

    • DOI

      10.1021/acs.biomac.7b01768

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 新規配列制御型グリコール酸ポリマーの生合成と構造解析2020

    • Author(s)
      梶川彩香、榊原早也果、荒井修造、大井俊彦、堀千明、松本謙一郎
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 微生物産生ブロック共重合体ポリマーの構造機能相関の解析2020

    • Author(s)
      佐藤 圭悟、影山 友基、藤井 隆之輔、松本 謙一郎
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] プラスチックの微生物合成:細胞の中で起こっていること2018

    • Author(s)
      松本謙一郎
    • Organizer
      アグリバイオ公開シンポジウム -植物バイオテクノロジーの最前線-
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] New insight into synthetic mechanism of bacterial polyhydroxyalkanoate for fine polymer structure control2018

    • Author(s)
      Matsumoto K
    • Organizer
      The 10th International Conference of Modification, Degradation and Stabilization of Polymers
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Copolymerization Kinetics of Engineered Polyhydroxyalkanoate Synthase in Production of 2-Hydroxyalkanoate-containing Polymers2018

    • Author(s)
      Matsumoto K
    • Organizer
      16th International Symposium on Biopolymers
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microbial synthesis of unusual polyhydroxyalkanoate (PHA) from lignocellulosic biomass2018

    • Author(s)
      Matsumoto K, Kaodya R, Takisawa K, Ooi T, Hori C, Yamada T, and Taguchi S
    • Organizer
      International Symposium on Biomass Refinery: From Biomass Crops to Chemicals and Fuels
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Patent(Industrial Property Rights)] ブロック共重合体、及びその製造方法2020

    • Inventor(s)
      松本謙一郎
    • Industrial Property Rights Holder
      松本謙一郎
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-034109
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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