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Theobromine, a primary methylxanthine in cacao beans, improves cognitive performance.

Research Project

Project/Area Number 17H01963
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits
Research InstitutionKanazawa University

Principal Investigator

SUGIMOTO Naotoshi  金沢大学, 医学系, 准教授 (80272954)

Co-Investigator(Kenkyū-buntansha) 紫藤 治  島根大学, 学術研究院医学・看護学系, 教授 (40175386)
谷内江 昭宏  金沢大学, 附属病院, 特任教授 (40210281)
片倉 賢紀  城西大学, 薬学部, 准教授 (40383179)
上田 善文  東京大学, 大学院理学系研究科(理学部), 特任研究員 (60391877)
宮本 まゆみ  島根大学, 学術研究院医学・看護学系, 講師 (80551746)
田中 正二  金沢大学, 保健学系, 助教 (70422657)
内田 薫  高崎健康福祉大学, 健康福祉学部, 助教 (30724132)
横山 忠史  金沢大学, 附属病院, 助教 (00837362)
少作 隆子  金沢大学, 保健学系, 教授 (60179025)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords食品 / 嗜好食品 / メチルキサンチン誘導体 / 抗老化作用 / 認知機能 / イメージング
Outline of Final Research Achievements

Theobromine (TB) is a primary methylxanthine found in cacao beans. Previous reports showed that habitual chocolate intake was related to cognitive performance and frequent chocolate consumption was significantly associated with better memory performances. Here we investigated whether theobromine acts on the cognitive and learning function in rats and human. Male Wistar rats received a normal diet or the same food with a 0.05% TB supplement, while healthy young subjects were intake dark chocolate or theobromine -free white chocolate.
The working memory in rats or human seemed to be improved by theobromine or dark chocolate ingestion, respectively. These results suggested that consumption of dark chocolate (theobromine) has sub-chronical beneficial effects on health to enhance cognitive functions.

Academic Significance and Societal Importance of the Research Achievements

子供から大人まで幅広く摂取される嗜好食品の代表がチョコレートである。チョコレートはメチルキサンチン誘導体であるテオブロミンを多く含み、その摂取が認知機能を亢進させることが明らかとなった。高齢化が進む中、認知症への取り組みは重要な課題である。本研究は、チョコレートの摂取が認知症の予防に貢献する可能性を示唆したことで、学術的にも社会的にも意義あるものである。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (27 results)

All 2021 2020 2019 2018 2017

All Journal Article (14 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 14 results,  Open Access: 13 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 10 results)

  • [Journal Article] Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells2021

    • Author(s)
      Sugimoto Naotoshi、Katakura Masanori、Matsuzaki Kentaro、Miyamoto Mayumi、Sumiyoshi Eri、Wada Taizo、Yachie Akihiro、Nakamura Hiroyuki、Shido Osamu
    • Journal Title

      Molecular Medicine Reports

      Volume: 23 Issue: 4 Pages: 1-8

    • DOI

      10.3892/mmr.2021.11889

    • NAID

      120007165535

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] テオブロミンの摂取が骨髄由来体性幹細胞の増殖および分化能に与える影響2020

    • Author(s)
      堀本 泰弘、中谷 祥恵、齋藤 彩果、片倉 賢紀、杉本 直俊、古旗 賢二
    • Journal Title

      Functional Food Research

      Volume: 16 Pages: 57-64

    • NAID

      130007899866

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Salivary Immunoglobulin A Secretion and Polymeric Ig Receptor Expression in the Submandibular Glands Are Enhanced in Heat-Acclimated Rats2020

    • Author(s)
      Matsuzaki Kentaro、Sugimoto Naotoshi、Islam Rafiad、Hossain Md Emon、Sumiyoshi Eri、Katakura Masanori、Shido Osamu
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 3 Pages: 815-815

    • DOI

      10.3390/ijms21030815

    • NAID

      120007002165

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Influence of Ultra-High Hydrostatic Pressurizing Brown Rice on Cognitive Functions and Mental Health of Elderly Japanese Individuals: A 2-Year Randomized and Controlled Trial2019

    • Author(s)
      KURODA Yoko、MATSUZAKI Kentaro、WAKATSUKI Harumi、SHIDO Osamu、HARAUMA Akiko、MORIGUCHI Toru、SUGIMOTO Hachiro、YAMAGUCHI Shuhei、YOSHINO Katsumi、HASHIMOTO Michio
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 65 Issue: Supplement Pages: S80-S87

    • DOI

      10.3177/jnsv.65.S80

    • NAID

      130007729768

    • ISSN
      0301-4800, 1881-7742
    • Year and Date
      2019-10-11
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-Term Ultra-High Hydrostatic Pressurized Brown Rice Intake Prevents Bone Mineral Density Decline in Elderly Japanese Individuals2019

    • Author(s)
      MATSUZAKI Kentaro、YANO Shozo、SUMIYOSHI Eri、SHIDO Osamu、KATSUBE Takuya、TABATA Mitsumasa、OKUDA Michiaki、SUGIMOTO Hachiro、YOSHINO Katsumi、HASHIMOTO Michio
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 65 Issue: Supplement Pages: S88-S92

    • DOI

      10.3177/jnsv.65.S88

    • NAID

      130007729765

    • ISSN
      0301-4800, 1881-7742
    • Year and Date
      2019-10-11
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sub-Chronic Consumption of Dark Chocolate Enhances Cognitive Function and Releases Nerve Growth Factors: A Parallel-Group Randomized Trial2019

    • Author(s)
      Sumiyoshi Eri、Matsuzaki Kentaro、Sugimoto Naotoshi、Tanabe Yoko、Hara Toshiko、Katakura Masanori、Miyamoto Mayumi、Mishima Seiji、Shido Osamu
    • Journal Title

      Nutrients

      Volume: 11 Issue: 11 Pages: 2800-2800

    • DOI

      10.3390/nu11112800

    • NAID

      120007002197

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Corticosterone inhibits the expression of cannabinoid receptor?1 and cannabinoid receptor agonist?induced decrease in cell viability in glioblastoma cells2019

    • Author(s)
      Sugimoto Naotoshi、Ishibashi Hiroaki、Ueda Yoshibumi、Nakamura Hiroyuki、Yachie Akihiro、Ohno?Shosaku Takako
    • Journal Title

      Oncology Letters

      Volume: 18 Pages: 1557-1563

    • DOI

      10.3892/ol.2019.10456

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Theobromine Improves Working Memory by Activating the CaMKII/CREB/BDNF Pathway in Rats2019

    • Author(s)
      Islam Rafiad、Matsuzaki Kentaro、Sumiyoshi Eri、Hossain Md Emon、Hashimoto Michio、Katakura Masanori、Sugimoto Naotoshi、Shido Osamu
    • Journal Title

      Nutrients

      Volume: 11 Issue: 4 Pages: 888-888

    • DOI

      10.3390/nu11040888

    • NAID

      120006901632

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heat attenuates sensitivity of mammalian cells to capsaicin.2019

    • Author(s)
      Sugimoto N, Matsuzaki K, Katakura M, Nakamura H, Ueda Y, Yachie A, Shido O.
    • Journal Title

      J Biochem Mol Toxicol.

      Volume: e22288 Issue: 5 Pages: 1-7

    • DOI

      10.1002/jbt.22288

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of chronic administration of arachidonic acid on the performance of learning and memory in aged rats.2019

    • Author(s)
      Inoue T, Hashimoto M, Katakura M, Hossain S, Matsuzaki K, Shido O.
    • Journal Title

      Food Nutr Res.

      Volume: 63 Issue: 0 Pages: 1-9

    • DOI

      10.29219/fnr.v63.1441

    • NAID

      120006901631

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chronic administration of theobromine inhibits mTOR signal in rats.2018

    • Author(s)
      Sugimoto N, Katakura M, Matsuzaki K, Sumiyoshi E, Yachie A, Shido O.
    • Journal Title

      Basic Clin Pharmacol Toxicol.

      Volume: 00 Issue: 5 Pages: 1-7

    • DOI

      10.1111/bcpt.13175

    • NAID

      120007002194

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct exposure to mild heat promotes proliferation and neuronal differentiation of neural stem/progenitor cells in vitro2017

    • Author(s)
      Hossain Md Emon、Matsuzaki Kentaro、Katakura Masanori、Sugimoto Naotoshi、Mamun Abdullah Al、Islam Rafiad、Hashimoto Michio、Shido Osamu
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 12 Pages: e0190356-e0190356

    • DOI

      10.1371/journal.pone.0190356

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of dietary n-3 fatty acids supplementation on fatty acid metabolism in atorvastatin-administered SHR.Cg-Leprcp/NDmcr rats, a metabolic syndrome model.2017

    • Author(s)
      Abdullah Al Mamun, Michio Hashimoto, Masanori Katakura, Yoko Tanabe, Satoru Tsuchikura, Shahdat Hossain, Osam Shido
    • Journal Title

      Biomed Pharmacother

      Volume: 85 Pages: 372-379

    • DOI

      10.1016/j.biopha.2016.11.038

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Neural progenitor cell proliferation in the hypothalamus is involved in acquired heat tolerance in long-term heat-acclimated rats2017

    • Author(s)
      Matsuzaki Kentaro、Katakura Masanori、Sugimoto Naotoshi、Hara Toshiko、Hashimoto Michio、Shido Osamu
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 6 Pages: e0178787-e0178787

    • DOI

      10.1371/journal.pone.0178787

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Long-Term Theobromine Supplementation Ameliorates Working Memory by Activating the CaMKII/CREB/BDNF Pathway in Rats2019

    • Author(s)
      Rafiad Islam, Kentaro Matsuzaki, Eri Sumiyoshi, Md Emon Hossain, Michio Hashimoto, Masanori Katakura, Naotoshi Sugimoto, Osamu Shido
    • Organizer
      第71回日本生理学会中国四国地方会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Possible improvement of cognitive function by long-term dark chocolate ingestion in young subjects.2019

    • Author(s)
      Eri Sumiyoshi, Kentaro Matsuzaki, Naotoshi Sugimoto, Yoko Tanabe, Toshiko Hara, Masanori Katakura, Mayumi Miyamoto, Osamu Shido
    • Organizer
      The 9th Federation of Asian and Oceanian Physiological Societies Congress (FAOPS2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Theobromine increases plasma cholesterol levels by increasing ABCA1 protein2019

    • Author(s)
      Natsuki Hiruma, Naotoshi Sugimoto, Kentaro Matsuzaki, Eri Sumiyoshi, Osamu Shido, Masanori Katakura
    • Organizer
      The 9th Federation of Asian and Oceanian Physiological Societies Congress (FAOPS2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of Ginger Powder Extracts on Actin Assembly In Vitro2018

    • Author(s)
      Naotoshi Sugimoto, Masanori Katakura, Kentaro Matsuzaki, Mayumi Miyamoto, Eri Sumiyoshi, Akihiro Yachie, Osamu Shido
    • Organizer
      EXPERIMENTAL BIOLOGY 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hypoxia downregulates cannabinoid receptors, upregulates COX-2, and attenuates cannabinoid receptor agonist-induced cell death in glioblastoma cells2018

    • Author(s)
      Naotoshi Sugimoto, Akihiro Yachie, Takako Ohno-Shosaku
    • Organizer
      8th International Congress of Pathophysiology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of long-term dark chocolate intake on cognitive function in healthy young subjects2018

    • Author(s)
      Eri Sumiyoshi, Kentaro Matsuzaki, Naotoshi Sugimoto, Yoko Tanabe, Toshiko Hara, Masanori Katakura, Mayumi Miyamoto, Osamu Shido
    • Organizer
      Europhysiology 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A THEOBROMINE-CONTAINING DIET STIMULATES CENTRAL CREB/BDNF PATHWAYS AND MOTOR LEARNING IN YOUNG MICE2018

    • Author(s)
      Naotoshi Sugimoto, Mitsugu Yoneda, Masanori Katakura, Kentaro Matsuzaki, Hayate Tanigami, Yuki Tabata, Takako Ohno-Shosaku, Osamu Shido, Akihiro Yachie
    • Organizer
      The 14th Asian Pan - Pacific Society of Pediatric Gastroenterology, Hepatology and Nutrition Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ginger powder extract prevents heat shock-induced cell death in mouse fibroblast cells2018

    • Author(s)
      Naotoshi Sugimoto, Masanori Katakura, Kentaro Matsuzaki, Mayumi Miyamoto, Eri Sumiyoshi, Akihiro Yachie, Osamu Shido
    • Organizer
      Pharmacology 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Theobromine improves working memory by activating CREB/BDNF pathway in rats.2018

    • Author(s)
      Islam Rafiad, Kentaro Matsuzaki, Naotoshi Sugimoto, Masanori Katakura, Eri Sumiyoshi, Michio Hashimoto, Osamu Shido
    • Organizer
      The 4th International Conference on Pharma-Food (ICPF 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Lipopolysaccharide increases the expression level of the aquaporin-4 water channel.2017

    • Author(s)
      Sugimoto N, Nishimura N, Ohnishi N, Matsumoto T, Kitaura T, Shido O, Yachie A.
    • Organizer
      Experimental Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] テオブロミンのマウス海馬神経幹細胞増殖に及ぼす影響2017

    • Author(s)
      井野綾音、片倉賢紀、杉林堅次、杉本直俊、松崎健太郎、紫藤治
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ラット由来培養神経幹細胞の分化に及ぼすテオブロミンの影響2017

    • Author(s)
      並木渓、片倉賢紀、杉林堅次、杉本直俊、松崎健太郎
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Ginger Powder Extracts activate MAP kinases in mouse fibroblast cells2017

    • Author(s)
      Naotoshi Sugimoto, Masanori Katakura, Kentaro Matsuzaki, Mayumi Miyamoto, Eri Sumiyoshi, Akihiro Yachie, Osamu Shido
    • Organizer
      British Pharmacological Society 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2022-01-27  

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