Establishment of "Contractile Muscle Cell Culture System" using Human-derived Satellite Cells
| Project/Area Number |
17H02076
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tohoku University |
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Principal Investigator |
Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
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| Co-Investigator(Kenkyū-buntansha) |
萩原 嘉廣 東北大学, 医学系研究科, 准教授 (90436139)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 骨格筋 / 筋細胞 / 運動 / 培養システム / 電気パルス / 筋疾患 / マイオカイン / 培養系 / フレイル / 細胞 / 特殊培養システム / サルコペニア / 生活習慣病 / 細胞診断 / 運動効果 / バイオテクノロジー / 培養細胞 / 再生医学 / 糖尿病・生活習慣病 |
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| Outline of Final Research Achievements |
This study aimed to establish an “in vitro exercise model” using human-derived muscle cells that often exhibit relatively poor contractile activity. By employing biomedical engineering techniques along with co-culture systems, we successfully established “in vitro exercise models” using (1) hybrid myotubes comprised of human myoblasts and murine C2C12 myoblasts as well as those using (2) murine 3T3L1 cells as a feeder for supporting development of human myotube contractile ability, that both exhibit vigorous contractile activity in response to electric pulse stimulation (EPS). Species-specific analyses allow us to separately evaluate contraction-inducible gene expressions and myokine secretions from human and mouse constituents. We also fabricated a culture insert chamber that one can efficiently deliver EPS to the myotubes. Thus, these inventions would allow us to maximize the use of the human myocytes obtained from biopsy samples, a very limited resource.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題の達成により確立されたヒト由来筋細胞を用いた「運動できる培養筋細胞系」を活用することにより、立ち後れている骨格筋を治療標的とする創薬研究に対して大きな貢献をもたらすことができると期待される。また、健常者のみならず、筋疾患患者より採取したヒト筋細胞をこの特殊培養系にて人為的な任意の収縮運動を負荷することにより、収縮能力の獲得過程や、運動応答性に惹起される各種の高次筋機能（マイオカイン類発現調節、アダプテーションや代謝能亢進など）を培養系において評価可能な「筋細胞高次機能診断システム」の将来的な構築が可能となった。
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] Unveiling the coupling of flotillin-1 and extracellular α-synuclein: relevance to dopamine transporter trafficking and Lewy body formation2019
Author(s)
Kobayashi J, Hasegawa T, Sugeno N, Yoshida S, Miki Y, Fukuda M, Kawahata I, Yamakuni T, Tomiyama A, Kawata Y, Ezura M, Kikuchi A, Baba T, Wakabayashi K, Takeda A, Aoki M
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Journal Title
FASEB Journal
Volume: 33
Issue: 9
Pages: 10240-10256
DOI
Related Report
Peer Reviewed
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[Journal Article] Yoshida S, Hagiwara Y, Tsuchiya M, Shinoda M, Koide M, Hatakeyama H, Chaweewannakorn C, Yano T, Sogi Y, Itaya N, Sekiguchi T, Yabe Y, Sasaki K, Kanzaki M, Itoi E.2018
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[Journal Article] Olfactory receptors are expressed in pancreatic beta-cells and promote glucose-stimulated insulin secretion.2018
Author(s)
Munakata Y, Yamada T, Imai J, Takahashi K, Tsukita S, Shirai Y, Kodama S, Asai Y, Sugisawa T, Chiba Y, Kaneko K, Uno K, Sawada S, Hatakeyama H, Kanzaki M, Miyazaki JI, Oka Y, Katagiri H
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 1499-1499
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration2018
Author(s)
Koide M, Hagiwara Y, Tsuchiya M, Kanzaki M, Hatakeyama H, Tanaka Y, Minowa T, Takemura T, Ando A, Sekiguchi T, Yabe Y, Itoi E.
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Journal Title
The Tohoku Journal of Experimental Medicine
Volume: 244
Issue: 1
Pages: 15-24
DOI
NAID
ISSN
0040-8727, 1349-3329
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Neuronal signals regulate obesity induced beta-cell proliferation by FoxM1 dependent mechanism.2017
Author(s)
Yamamoto J, Imai J, Izumi T, Takahashi H, Kawana Y, Takahashi K, Kodama S, Kaneko K, Gao J, Uno K, Sawada S, Asano T, Kalinichenko VV, Susaki EA, Kanzaki M, Ueda HR, Ishigaki Y, Yamada T, Katagiri H
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Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 1930-1930
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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