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Establishment of "Contractile Muscle Cell Culture System" using Human-derived Satellite Cells

Research Project

Project/Area Number 17H02076
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTohoku University

Principal Investigator

Kanzaki Makoto  東北大学, 医工学研究科, 准教授 (10272262)

Co-Investigator(Kenkyū-buntansha) 萩原 嘉廣  東北大学, 医学系研究科, 准教授 (90436139)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords骨格筋 / 筋細胞 / 運動 / 培養システム / 電気パルス / 筋疾患 / マイオカイン / 培養系 / フレイル / 細胞 / 特殊培養システム / サルコペニア / 生活習慣病 / 細胞診断 / 運動効果 / バイオテクノロジー / 培養細胞 / 再生医学 / 糖尿病・生活習慣病
Outline of Final Research Achievements

This study aimed to establish an “in vitro exercise model” using human-derived muscle cells that often exhibit relatively poor contractile activity. By employing biomedical engineering techniques along with co-culture systems, we successfully established “in vitro exercise models” using (1) hybrid myotubes comprised of human myoblasts and murine C2C12 myoblasts as well as those using (2) murine 3T3L1 cells as a feeder for supporting development of human myotube contractile ability, that both exhibit vigorous contractile activity in response to electric pulse stimulation (EPS). Species-specific analyses allow us to separately evaluate contraction-inducible gene expressions and myokine secretions from human and mouse constituents. We also fabricated a culture insert chamber that one can efficiently deliver EPS to the myotubes. Thus, these inventions would allow us to maximize the use of the human myocytes obtained from biopsy samples, a very limited resource.

Academic Significance and Societal Importance of the Research Achievements

本研究課題の達成により確立されたヒト由来筋細胞を用いた「運動できる培養筋細胞系」を活用することにより、立ち後れている骨格筋を治療標的とする創薬研究に対して大きな貢献をもたらすことができると期待される。また、健常者のみならず、筋疾患患者より採取したヒト筋細胞をこの特殊培養系にて人為的な任意の収縮運動を負荷することにより、収縮能力の獲得過程や、運動応答性に惹起される各種の高次筋機能(マイオカイン類発現調節、アダプテーションや代謝能亢進など)を培養系において評価可能な「筋細胞高次機能診断システム」の将来的な構築が可能となった。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (78 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (8 results) Journal Article (27 results) (of which Int'l Joint Research: 11 results,  Peer Reviewed: 21 results,  Open Access: 9 results) Presentation (35 results) (of which Int'l Joint Research: 21 results,  Invited: 9 results) Remarks (7 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] ラバル大学・分子医学(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ストラスブール大学・糖尿病研究所(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ミシガン大学・運動学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Michigan(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] LENS-Eur. Lab. Non-linear Spectroscopy/University of Florence/INO-National Institute of Optics(イタリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Strasbourg University/CEED (Centre European Etude de Diabetes)(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Michigan University(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] CEED (Centre European Etude de Diabetes)(France)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Characterization of middle-molecule introduction into cells using mm-scale discharge in saline2020

    • Author(s)
      R. Honda, S. Sasaki, K. Takashima, M. Kanzaki, T. Sato, and T. Kaneko
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: 4 Pages: 040904-040904

    • DOI

      10.35848/1347-4065/ab7d7e

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of optical resolution photoacoustic microscopy with sub-micron lateral resolution for visualization of cells and their structures2020

    • Author(s)
      Shintate Ryo、Morino Taisuke、Kawaguchi Kota、Nagaoka Ryo、Kobayashi Kazuto、Kanzaki Makoto、Saijo Yoshifumi
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: SK Pages: SKKE11-SKKE11

    • DOI

      10.35848/1347-4065/ab840e

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 免疫伝達物質cGAMPのトランスポーターSLC19Aの同定2020

    • Author(s)
      神﨑 展
    • Journal Title

      実験医学1月号

      Volume: 38(1) Pages: 60-61

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Exercise‐evoked intramuscular neutrophil‐endothelial interactions support muscle performance and GLUT4 translocation: a mouse gnawing model study2019

    • Author(s)
      Chaweewannakorn Chayanit、Nyasha Mazvita R.、Chen Weijian、Sekiai Shigenori、Tsuchiya Masahiro、Hagiwara Yoshihiro、Bouzakri Karim、Sasaki Keiichi、Kanzaki Makoto
    • Journal Title

      The Journal of Physiology

      Volume: 598 Issue: 1 Pages: 101-122

    • DOI

      10.1113/jp278564

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In vitro exercise model using contractile human and mouse hybrid myotubes2019

    • Author(s)
      Chen Weijian、Nyasha Mazvita R.、Koide Masashi、Tsuchiya Masahiro、Suzuki Naoki、Hagiwara Yoshihiro、Aoki Masashi、Kanzaki Makoto
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 11914-11914

    • DOI

      10.1038/s41598-019-48316-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sparc, an EPS-induced gene, modulates the extracellular matrix and mitochondrial function via ILK/AMPK pathways in C2C12 cells2019

    • Author(s)
      Melouane Aicha、Yoshioka Mayumi、Kanzaki Makoto、St-Amand Jonny
    • Journal Title

      Life Sciences

      Volume: 229 Pages: 277-287

    • DOI

      10.1016/j.lfs.2019.05.070

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Unveiling the coupling of flotillin-1 and extracellular α-synuclein: relevance to dopamine transporter trafficking and Lewy body formation2019

    • Author(s)
      Kobayashi J, Hasegawa T, Sugeno N, Yoshida S, Miki Y, Fukuda M, Kawahata I, Yamakuni T, Tomiyama A, Kawata Y, Ezura M, Kikuchi A, Baba T, Wakabayashi K, Takeda A, Aoki M
    • Journal Title

      FASEB Journal

      Volume: 33 Issue: 9 Pages: 10240-10256

    • DOI

      10.1096/fj.201802051r

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biomechanics of C2C12 Cells Observed with Cellular Resolution Scanning Acoustic Microscope Combined with Optical Microscope *2019

    • Author(s)
      Hirano Ryo、Kanzaki Makoto、Arakawa Mototaka、Hermawan Norma、Kobayashi Kazuto、Saijo Yoshifumi
    • Journal Title

      Ann Int Conf of IEEE Eng in Med and Biol

      Volume: 8857008 Pages: 4828-4831

    • DOI

      10.1109/embc.2019.8857008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of inflammasome activation via elevation of uric acid level in nociception in a mouse model of muscle pain2019

    • Author(s)
      Yoshida Shinichirou、Hagiwara Yoshihiro、Tsuchiya Masahiro、Shinoda Masamichi、Koide Masashi、Hatakeyama Hiroyasu、Chaweewannakorn Chayanit、Suzuki Kazuaki、Yano Toshihisa、Sogi Yasuhito、Itaya Nobuyuki、Sekiguchi Takuya、Yabe Yutaka、Sasaki Keiichi、Kanzaki Makoto、Itoi Eiji
    • Journal Title

      Molecular Pain

      Volume: 15 Pages: 1-16

    • DOI

      10.1177/1744806919858797

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ヒト筋衛星細胞を用いた「in vitro Exerciseモデル」の構築2019

    • Author(s)
      神﨑 展
    • Journal Title

      Medical Science Digest

      Volume: 45(6) Pages: 358-361

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 2型糖尿病治療薬メトホルミンの肝臓における真なる薬理機序2019

    • Author(s)
      神﨑 展
    • Journal Title

      実験医学

      Volume: 37 (1) Pages: 60-61

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effects of Acute Exercise Combined With Calorie Restriction Initiated Late-in-Life on Insulin Signaling, Lipids, and Glucose Uptake in Skeletal Muscle From Old Rats2018

    • Author(s)
      Oki Kentaro、Arias Edward B、Kanzaki Makoto、Cartee Gregory D
    • Journal Title

      The Journals of Gerontology: Series A

      Volume: in press Issue: 2 Pages: 207-217

    • DOI

      10.1093/gerona/gly222

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities2018

    • Author(s)
      Hatakeyama Hiroyasu、Morino Taisuke、Ishii Takuya、Kanzaki Makoto
    • Journal Title

      Journal of Biological Chemistry

      Volume: 294 Issue: 4 Pages: 1161-1172

    • DOI

      10.1074/jbc.ra118.004614

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prior treatment with the AMPK activator AICAR induces subsequently enhanced glucose uptake in isolated skeletal muscles from 24 month-old rats2018

    • Author(s)
      Oki Kentaro、Arias Edward B、Kanzaki Makoto、Cartee Gregory D
    • Journal Title

      Appl. Physiol. Nutr. Metab.

      Volume: 印刷中 Issue: 8 Pages: 795-805

    • DOI

      10.1139/apnm-2017-0858

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation2018

    • Author(s)
      Kuniaki Nagamine, Hirotaka Sato, Hiroyuki Kai, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 2253-2253

    • DOI

      10.1038/s41598-018-20729-y

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Yoshida S, Hagiwara Y, Tsuchiya M, Shinoda M, Koide M, Hatakeyama H, Chaweewannakorn C, Yano T, Sogi Y, Itaya N, Sekiguchi T, Yabe Y, Sasaki K, Kanzaki M, Itoi E.2018

    • Author(s)
      Involvement of neutrophils and interleukin-18 in nociception in a mouse model of muscle pain.
    • Journal Title

      Mol Pain.

      Volume: -

    • DOI

      10.1177/1744806918757286

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Olfactory receptors are expressed in pancreatic β-cells and promote glucose-stimulated insulin secretion2018

    • Author(s)
      Munakata Y, Yamada T, Imai J, Takahashi K, Tsukita S, Shirai Y, Kodama S, Asai Y, Sugisawa T, Chiba Y, Kaneko K, Uno K, Sawada S, Hatakeyama H, Kanzaki M, Miyazaki JI, Oka Y, Katagiri H
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1499-1499

    • DOI

      10.1038/s41598-018-19765-5

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration2018

    • Author(s)
      Koide M, Hagiwara Y, Tsuchiya M, Kanzaki M, Hatakeyama H, Tanaka Y, Minowa T, Takemura T, Ando A, Sekiguchi T, Yabe Y, Itoi E.
    • Journal Title

      The Tohoku Journal of Experimental Medicine

      Volume: 244 Issue: 1 Pages: 15-24

    • DOI

      10.1620/tjem.244.15

    • NAID

      130006287941

    • ISSN
      0040-8727, 1349-3329
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells2018

    • Author(s)
      Gardini Lucia、Calamai Martino、Hatakeyama Hiroyasu、Kanzaki Makoto、Capitanio Marco、Pavone Francesco Saverio
    • Journal Title

      Methods in molecular biology

      Volume: 1841 Pages: 425-448

    • DOI

      10.1007/978-1-4939-8591-3_26

    • ISBN
      9781493985906, 9781493985913
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Neutrophils Provide a Favorable IL-1-Mediated Immunometabolic Niche that Primes GLUT4 Translocation and Performance in Skeletal Muscles2018

    • Author(s)
      Tsuchiya Masahiro、Sekiai Shigenori、Hatakeyama Hiroyasu、Koide Masashi、Chaweewannakorn Chayanit、Yaoita Fukie、Tan-No Koichi、Sasaki Keiichi、Watanabe Makoto、Sugawara Shunji、Endo Yasuo、Itoi Eiji、Hagiwara Yoshihiro、Kanzaki Makoto
    • Journal Title

      Cell Reports

      Volume: 23 Issue: 8 Pages: 2354-2364

    • DOI

      10.1016/j.celrep.2018.04.067

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] イタコン酸によるマクロファージ免疫代謝制御系の解明2018

    • Author(s)
      神﨑 展
    • Journal Title

      実験医学

      Volume: 36 (11) Pages: 1878-1879

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Roles of IL-1α/β in regeneration of cardiotoxin-injured muscle and satellite cell function2018

    • Author(s)
      Chaweewannakorn Chayanit、Tsuchiya Masahiro、Koide Masashi、Hatakeyama Hiroyasu、Tanaka Yukinori、Yoshida Shinichirou、Sugawara Shunji、Hagiwara Yoshihiro、Sasaki Keiichi、Kanzaki Makoto
    • Journal Title

      Am. J. Physiol. Regul. Integr. Comp. Physiol.

      Volume: 印刷中 Issue: 1 Pages: R90-R103

    • DOI

      10.1152/ajpregu.00310.2017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 自然免疫DNAセンサー(cGAS)による細胞老化の制御2018

    • Author(s)
      神﨑 展
    • Journal Title

      実験医学

      Volume: 36(1) Pages: 60-61

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Neuronal signals regulate obesity induced β-cell proliferation by FoxM1 dependent mechanism2017

    • Author(s)
      Yamamoto J, Imai J, Izumi T, Takahashi H, Kawana Y, Takahashi K, Kodama S, Kaneko K, Gao J, Uno K, Sawada S, Asano T, Kalinichenko VV, Susaki EA, Kanzaki M, Ueda HR, Ishigaki Y, Yamada T, Katagiri H
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 1930-1930

    • DOI

      10.1038/s41467-017-01869-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 3D electrochemical and ion current imaging using scanning electrochemical-scanning ion conductance microscopy2017

    • Author(s)
      4)Takahashi, Y.; Ida, H.; Matsumae, Y.; Komaki, H.; Zhou, Y.; Kumatani, A.; Kanzaki, M.; Shiku, H.; Matsue, T.
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 19 Issue: 39 Pages: 26728-26733

    • DOI

      10.1039/c7cp05157c

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterotypic endosomal fusion as an initial trigger for insulin‐induced glucose transporter 4 (GLUT4) translocation in skeletal muscle2017

    • Author(s)
      Hiroyasu Hatakeyama, Makoto Kanzaki
    • Journal Title

      J. Physiol.

      Volume: 595(16) Issue: 16 Pages: 5603-5621

    • DOI

      10.1113/jp273985

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] タンパク質の品質管理と寿命決定-CHIPによるインスリン受容体の恒常性制御-2017

    • Author(s)
      神﨑 展
    • Journal Title

      実験医学

      Volume: 35(11) Pages: 57-58

    • Related Report
      2017 Annual Research Report
  • [Presentation] 液相中プラズマ直接照射による薬剤模擬分子導入の作用機序に関する研究2020

    • Author(s)
      本田 竜介,佐々木 渉太,高島 圭介,神崎 展,佐藤 岳彦,金子 俊郎
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Dominant Factors of In-Liquid Micro Plasma for Drug Introduction into Cells2019

    • Author(s)
      R. Honda , S. Sasaki , K. Takashima , M. Kanzaki , T. Sato and T. Kaneko
    • Organizer
      The 10th International Workshop on Microplasmas (IWM-10)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Drug introduction into cells using direct exposure of gas-liquid interfacial plasmas2019

    • Author(s)
      R. Honda , S. Sasaki , K. Takashima , M. Kanzaki , T. Sato and T. Kaneko
    • Organizer
      24th International Symposium on Plasma Chemistry
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The molecule introduction into cells by in-liquid plasma irradiation and investigation of the key factor for introduction2019

    • Author(s)
      R. Honda , S. Sasaki , K. Takashima , M. Kanzaki , T. Sato , and T. Kaneko
    • Organizer
      The 6th Japan-Taiwan Workshop on Plasma Life Science and Technology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Short-lived reactive oxygen nitrogen species continuously generated in plasma-exposed solution activates TRP channels and drug uptake2019

    • Author(s)
      S. Sasaki , M. Kawase , M. Kanzaki , T. Kaneko
    • Organizer
      The Joint Conference of XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-Solution Type Plasma Device for Highly-Efficient Drug Transfer2019

    • Author(s)
      R. Honda , S. Sasaki , K. Takashima , M. Kanzaki , T. Sato , and T. Kaneko
    • Organizer
      The Joint Conference of XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gas-Liquid Interfacial Plasmas Yielding Short-Lived Reactive Species for Medical and Agricultural Applications2019

    • Author(s)
      T. Kaneko , S. Sasaki , K. Takashima , R. Honda , M. Kanzaki
    • Organizer
      The 12th Asian-European International Conference on Plasma Surface Engineering
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 液相中プラズマによる分子導入因子の探索2019

    • Author(s)
      R. Honda , S. Sasaki , K. Takashima , M. Kanzaki , T. Sato , and T. Kaneko
    • Organizer
      第29回日本MRS年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Gas-Liquid Interfacial Plasmas Generating Short-Lived Reactive Species for Drug/Gene Transfer into Living Cells2019

    • Author(s)
      T. Kaneko , S. Sasaki , R. Honda , M. Kanzaki
    • Organizer
      The 42nd PhotonIcs & Electromagnetics Research Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Implication of a new protein in the insulin vesicle trafficking2019

    • Author(s)
      Kanzaki, M.
    • Organizer
      EuroDIA meeting 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fractalkine/CX3CL1 serves as a key factor organizing the neutrophil-mediated favorable immunometabolic niche in working skeletal muscles2019

    • Author(s)
      Kanzaki, M., Chaweewannakorn, C. Tsuchiya, M. Sasaki, K.
    • Organizer
      Cell Symposia, Exercise Metabolism
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 膵β細胞のインスリン分泌とアクチンダイナミクス制御におけるMARCKSタンパクの関与2019

    • Author(s)
      石井琢也、Allan Langlois, 小島大輝、畠山裕康、Karim Bouzakri, 神﨑 展
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 運動骨格筋内微小領域(運動筋ニッチ)における好中球の役割2019

    • Author(s)
      神﨑 展
    • Organizer
      第5回日本筋学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 液相中プラズマの安定生成と薬剤分子局所導入2019

    • Author(s)
      R. Honda,S. Sasaki,K. Takashima,M. Kanzaki,T. Sato,and T. Kaneko
    • Organizer
      第36回 プラズマプロセシング研究会/第31回 プラズマ材料科学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] プラズマ照射緩衝液を用いた培養細胞に対する短寿命活性種の持続供給2019

    • Author(s)
      佐々木 渉太,鄭 悦星,神崎 展,金子 俊郎
    • Organizer
      仙台“プラズマフォーラム”
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多角的光学イメージング計測により解明したマウス骨格筋繊維におけるGLUT4輸送制御とインスリン初期作用2018

    • Author(s)
      畠山裕康・神崎 展
    • Organizer
      第61回日本糖尿病学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大気圧プラズマ反応性 Ca2+ 透過性チャンネルの同定2018

    • Author(s)
      瀬将義・川口航汰・畠山裕康・ 佐々木渉太・,金子俊郎・神﨑 展
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Roles of neutrophils and IL-1 in intramuscular immunometabolic niche for priming GLUT4 translocation during exercise2018

    • Author(s)
      Kanzaki Makoto, Sekiai Shigenori, Hatakeyama Hiroyasu, Chaweewannakorn Chayanit, Sasaki Keiichi, Hagiwara Yoshihiro, Itoi Eiji, Tsuchiya Masahiro
    • Organizer
      The 54th EASD (European Association for the Study of Diabetes) meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Introduction of drug simulated molecules into adherent cells using in-liquid plasmas2018

    • Author(s)
      R. Honda,S. Sasaki,K. Takashima,M. Kanzaki,T. Sato,and T. Kaneko
    • Organizer
      The 6th International Workshop and the 5th International Mini Workshop on Solution Plasma and Molecular Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gas-Liquid Interfacial Plasmas for Medical and Agricultural Applications2018

    • Author(s)
      T. Kaneko,K. Takashima,S. Sasaki,R. Honda,K. Nihei,M. Kanzaki,and T. Sato
    • Organizer
      The 6th International Workshop and the 5th International Mini Workshop on Solution Plasma and Molecular Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Investigation of Chemical Components in Plasma-exposed Organic Buffer for Drug Transfer Application2018

    • Author(s)
      S. Sasaki,Y. Zheng,M. Kanzaki,and T. Kaneko
    • Organizer
      7th International Conference on Plasma Medicine (ICPM7)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cell Membrane Transport Activated by Gas-Liquid Interfacial Plasmas for Future-Oriented Gene/Drug Transfer Device2018

    • Author(s)
      T. Kaneko,S. Sasaki,and M. Kanzaki
    • Organizer
      7th International Conference on Plasma Medicine (ICPM7)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 気液界面プラズマ生成複合刺激による細胞膜輸送促進の機構解明2018

    • Author(s)
      金子 俊郎,佐々木 渉太,神崎 展
    • Organizer
      第40回日本光医学・光生物学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 液相中プラズマによる薬剤模擬分子導入の作用機序の探索2018

    • Author(s)
      本田 竜介,佐々木 渉太,高島 圭介,神崎 展,佐藤 岳彦,金子 俊郎
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Roles of charged particles and reactive species on cell membrane permeabilization induced by atmospheric-pressure plasma irradiation2018

    • Author(s)
      佐々木 渉太,神崎 展,保苅 雄太郎,冨並 香菜子,目代 貴之,金高 弘恭,金子 俊郎
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Continuous release of short-lived species induced by plasma irradiation and its application in drug delivery2018

    • Author(s)
      S. Sasaki,Y. Zheng,M. Kanzaki,and T. Kaneko
    • Organizer
      2nd Asia-Pacific Conference on Plasma Physics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of in-liquid plasma on enhancement of cell membrane permeability2018

    • Author(s)
      R. Honda,S. Sasaki,K. Takashima,M. Kanzaki,T. Sato,and T. Kaneko
    • Organizer
      2nd Asia-Pacific Conference on Plasma Physics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プラズマ照射緩衝液中で活性化されるラジカル連鎖反応の解明2018

    • Author(s)
      佐々木 渉太,鄭 悦星,本田 竜介,高島 圭介,神崎 展,金子 俊郎
    • Organizer
      第35回 プラズマ・核融合学会 年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Effects of short-lived reactive species generated by non-thermal plasma on TRP channels and organic cation uptake2018

    • Author(s)
      S. Sasaki,M. Kawase,M. Kanzaki,and T. Kaneko
    • Organizer
      The 49th NIPS International Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Gas-Liquid Interfacial Plasma Devices for Medical and Agricultural Applications2018

    • Author(s)
      T. Kaneko,S. Sasaki,K. Takashima,R. Honda,K. Nihei,M. Kanzaki,and T. Sato
    • Organizer
      The 5th Taiwan-Japan Plasma Life Science and Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 大気圧プラズマによってトリガされる短寿命活性種の持続放出2018

    • Author(s)
      S. Sasaki,Y. Zheng,M. Kanzaki,and T. Kaneko
    • Organizer
      第28回 日本MRS年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 気液界面プラズマにおける細胞膜輸送増進短寿命活性種2018

    • Author(s)
      T. Kaneko,S. Sasaki,R. Honda,Y. Zheng,T. Mokudai,H. Kanetaka and M. Kanzaki
    • Organizer
      第28回 日本MRS年次大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Roles of IL-1alpha/beta and neutrophils in GLUT4 translocation and muscle performance during exercise2017

    • Author(s)
      Chaweewannakorn C., Sekiai, S., Tsuchiya M., Koide M., Hatakeyama H., Sasaki, K., Hagiwara Y., Kanzaki M.
    • Organizer
      FASEB Summer Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Roles of Interleukin-1 in the regulation of myoblast fusion and actin dynamics2017

    • Author(s)
      Chaweewannakorn C., Hatakeyama H.,Tsuchiya M., Hagiwara Y., Koide M., Yoshida S., Kanzaki M., Sasaki K.
    • Organizer
      American Society for Cell Biology meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of heterotypic endosomal fusion as an initial trigger for insulin-induced GLUT4 translocation in skeletal muscle revealed by comprehensive imaging analyses of GLUT4 behavior2017

    • Author(s)
      Hatakeyama H and Kanzaki M
    • Organizer
      FASEB Summer Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] ORCID: Makoto Kanzaki

    • URL

      https://orcid.org/0000-0002-6884-2955

    • Related Report
      2019 Annual Research Report
  • [Remarks] Research Map: Makoto Kanzaki

    • URL

      https://researchmap.jp/makotokanzaki

    • Related Report
      2019 Annual Research Report
  • [Remarks] Scopus: Makoto Kanzaki

    • URL

      https://www.scopus.com/authid/detail.uri?authorId=7006880707

    • Related Report
      2019 Annual Research Report
  • [Remarks] Resarcher ID: Makoto Kanzaki

    • URL

      https://publons.com/researcher/1489749/makoto-kanzaki/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 東北大学大学院医工学研究科・神﨑研ホームページ

    • URL

      http://www.ecei.tohoku.ac.jp/kanzaki/index.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] ResearchGate

    • URL

      https://www.researchgate.net/profile/Makoto_Kanzaki/

    • Related Report
      2018 Annual Research Report
  • [Remarks] Research Gate

    • URL

      https://www.researchgate.net/profile/Makoto_Kanzaki/

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞培養用インサート及び電気刺激用培養装置2019

    • Inventor(s)
      神崎 展
    • Industrial Property Rights Holder
      神崎 展
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-033091
    • Filing Date
      2019
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-05-20  

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