Oxygen imaging of 3D living cells using multi-color oxygen sensing probes
| Project/Area Number |
17H02078
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
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| Keywords | りん光 / イリジウム錯体 / りん光寿命 / イメージング / 酸素 / 細胞 / 組織 / スフェロイド / 可視化 |
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| Outline of Final Research Achievements |
In this study, we designed and synthesized intracellular oxygen probes based on phosphorescent iridium complexes for sensing oxygen levels into 3D-supheroides by using confocal phosphorescence lifetime imaging microscopy (PLIM). Moreover, ratiometric molecular probes consisting of a green fluorophore and a deep red phosphor connected by oligo arginine linkers were developed. We succeed the detection and visualization of intracellular oxygen levels of 3D-supheroides and living tissues using our probes and PLIM system.
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| Academic Significance and Societal Importance of the Research Achievements |
酸素は，我々が生命活動を維持するために必要不可欠な分子です。本研究では，組織モデルとして良く利用されている細胞塊（スフェロイド）内の酸素レベルを明らかにする方法論の開発に取り組みました。得られた研究成果から，スフェロイド内部の細胞は，外側の細胞に比べてより低酸素環境に陥っていることが分かりました。この知見は，がん腫瘍など，細胞が異常増殖し血流が不十分な組織内の酸素レベルを明らかにすることに繋がり，がんの発見，診断，治療法の開発に貢献することができます。
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] Synthesis, Structures, and Properties of Neutral and Radical Cationic S,C,C-Bridged Triphenylamine2020
Author(s)
Shin-ichiro Kato, Takanori Matsuoka, Shuichi Suzuki, Motoko S. Asano, Toshitada Yoshihara, Seiji Tobita, Taisuke Matsumoto, Chitoshi Kitamura
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Journal Title
Org. Lett.
Volume: 22
Issue: 2
Pages: 734-738
DOI
Related Report
Peer Reviewed
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