Biohybrid system composed of function devices for cell metabolism analysis

Research Project

Project/Area Number	17H02091
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Biomedical engineering/Biomaterial science and engineering
Research Institution	Ritsumeikan University
Principal Investigator	Satoshi Konishi 立命館大学, 理工学部, 教授 (50288627)
Co-Investigator(Kenkyū-buntansha)	小林大造立命館大学, 理工学部, 准教授 (20557433) 橋本健志立命館大学, スポーツ健康科学部, 教授 (70511608) 服部浩二立命館大学, 立命館グローバル・イノベーション研究機構, 准教授 (60409670)
Project Period (FY)	2017-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000) Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords	マイクロ・ナノデバイス / マイクロマシン / ナノバイオ
Outline of Final Research Achievements	This study developed a biohybrid system that incorporates a sampling device and functional devices such as biochemical sensors for cells cultured on chips. We constructed a system that incorporated the sampling device and biosensor devices introduced in this project into the dish system. The biohybrid system was applied to fat cells and skeletal muscle cells with the aim of the operation verification. Electrical stimulation evaluation experiments on skeletal muscle cells as well as the drug stimulation evaluation experiments for fat cells were successfully executed by the constructed system. It is expected that the application development of the biohybrid system constructed in this study is expected with confidence.
Academic Significance and Societal Importance of the Research Achievements	本研究では、チップ上に培養した細胞に対して、代謝物の回収デバイスや生化学センサ等の機能デバイスを組込んだバイオハイブリッドシステムの構築を進めた。薬剤投与や電気刺激等の外的刺激入力制御と出力となる代謝物の定期的な回収分析が可能なシステムを構築は、培養細胞組織の高頻度評価や自動化、さらにはバイオリズムを有する脂肪細胞等の細胞代謝現象の解明研究、さらには機能特性の変容現象の解明研究が可能となる。