Construction of flow culture system of 3D-Cancer-stromal Tissues for Evaluation of Interaction Between Cancer cells and Immunocytes
| Project/Area Number |
17H02099
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井上 正宏 京都大学, 医学研究科, 特定教授 (10342990)
西原 広史 慶應義塾大学, 医学部(信濃町), 教授 (50322805)
狩野 光伸 岡山大学, ヘルスシステム統合科学研究科, 教授 (80447383)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 三次元組織 / 腫瘍 / 間質 / 灌流培養 |
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| Outline of Final Research Achievements |
In this study, three-dimensional (3D)-cancer-stromal tissues containing immunocytes for flow culture system has been constructed by cell accumulation technology. Cancer tissue-originated spheroid (CTOS) has been embedded in the 3D tissues together with monocyte-derived dendritic cells for evaluation of CTOS-immunocyte interaction. Furthermore, sacrifacial gelan gum allowed construction of small blood vessel structure in the 3D tissues for flow culture. We also found that norteworthy synergistic effect of anticancer drugs and molecular targeting drugs has been evaluated by the 3D cancer-stromal tissues. Accordingly, this study has been successfully achieved as schedule.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で構築した3次元腫瘍－間質組織体は、がん個別化医療における最適治療薬の探索に有用であると期待される。がん細胞は患者一人一人で性質が異なるため、最適な治療方法、治療薬剤を見つけることが困難である。そこで、患者由来がん細胞を用いて本研究で見出された3次元組織体を作製することで、患者がん細胞の性質を反映して培養できるため、最適な治療薬剤のスクリーニングに有用であると期待される。従って、本研究成果の更なる発展により、がん個別化医療の実現が期待される。
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Report
(4 results)
Research Products
(69 results)
