Development of cell-specific DDS for biopharmaceuticals using a novel human-derived membrane penetration-enhancing peptide
| Project/Area Number |
17H02103
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Keio University |
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Principal Investigator |
DOI Nobuhide 慶應義塾大学, 理工学部(矢上), 教授 (50327673)
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| Co-Investigator(Kenkyū-buntansha) |
藤原 慶 慶應義塾大学, 理工学部(矢上), 講師 (20580989)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 抗体 / ペプチド / 核酸 / ドラッグデリバリーシステム / 細胞膜透過 / 蛋白質 / 癌 / バイオテクノロジー / 共焦点顕微鏡 |
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| Outline of Final Research Achievements |
Biopharmaceuticals consisting of antibodies, peptides or nucleic acids, which have high specificity and affinity for target molecules, are attracting attention as the ultimate molecular targeted drugs with less side effects and higher therapeutic effects than conventional drugs consisting of low-molecular compounds. However, biopharmaceuticals have a drawback of low cell membrane permeability, and thus development of an efficient drug delivery system (DDS) for biopharmaceuticals has been required. In this study, we discovered a membrane penetration-enhancing peptide for efficient cytoplasmic delivery of antibodies, peptides, and nucleic acids. In addition, as a basic research, we analyzed the membrane permeation mechanism.
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| Academic Significance and Societal Importance of the Research Achievements |
我々が発見した膜透過促進ペプチドはヒト天然タンパク質由来であり免疫原性の懸念が少ないという利点もあることから、この独自の膜透過促進ペプチドを連結した抗体／ペプチド／核酸がヒト細胞の細胞質に効率よく送達されるという本研究の成果は、将来的に、細胞内の疾患標的に作用する新しい抗体医薬の開発や、ペプチドや核酸からなる中分子医薬の効率的なデリバリーに応用することが期待できる。これにより、我が国のバイオ創薬技術の基盤強化、ひいては国民の医療・福祉の向上に大きく貢献することが期待できる。
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases.2020
Author(s)
Goto, H., Kanai, Y., Yotsui, A., Shimokihara, S., Shitara, S., Oyobiki, R., Fujiwara, K., Watanabe, T., Einaga, Y., Matsumoto, Y., Miki, N., Doi, N.
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Journal Title
Lab Chip
Volume: 20
Issue: 4
Pages: 852-861
DOI
Related Report
Peer Reviewed
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[Journal Article] Human-derived fusogenic peptides for the intracellular delivery of proteins.2017
Author(s)
Sudo, K., Niikura, K., Iwaki, K., Kohyama, S., Fujiwara, K., Doi, N.
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Journal Title
J. Control. Release
Volume: 255
Pages: 1-11
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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