| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
Recently, the muscle-specific protein myoglobin (Mb) has been reported to interact with complex IV to augment mitochondrial (Mito) respiratory capacity in skeletal muscles. The present study has aimed at to clarify the localization of Mb within Mito.
The isolated Mit was treated with the sucrose density-gradient centrifugation or proteinase K (PK), osmotic shock (OS), and TriX in order to digest proteins on the outer membrane and in the intramembrane. Combining treatment with PK, OS and TriX allowed immunoblotting detection of the Mito proteins localized in specific regions of Mit. Mb was detected with either PK or OS treatment, , suggesting that Mb associated with the inner membrane (intramembrane side, not matrix side). The endurance training increased Mb content but did not change Mb content in the Mito. However, the observation might imply that the dynamic flux of Mb from cytosol to Mito has greater importance than just the amount found in Mito.
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