| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
The enormous dynamic concentration range of plasma proteins of interest has made direct proteomic profiling of plasma one of the most challenging tasks faced in contemporary analytical biochemistry. We developed a novel differential solubilization method, which we call the DS method, for extracting peptides from plasma. The DS method has high extraction efficiency as compared to conventional peptide enrichment methods, which include organic solvent precipitation and ultrafiltration.In this study, we proposed new candidate bioactive peptides by combining this peptide analysis method with functional analyses and the in silico peptide discovery method.
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