Discovery of novel bioactive peptides using plasma peptidomics technology.
| Project/Area Number |
17H02206
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
七里 眞義 北里大学, 医学部, 教授 (10206097)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | プロテオミクス / ペプチドミクス / 血漿 / 生理活性ペプチド / ペプチド―ム / native peptide / 血液 / プロテオーム |
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| Outline of Final Research Achievements |
The enormous dynamic concentration range of plasma proteins of interest has made direct proteomic profiling of plasma one of the most challenging tasks faced in contemporary analytical biochemistry. We developed a novel differential solubilization method, which we call the DS method, for extracting peptides from plasma. The DS method has high extraction efficiency as compared to conventional peptide enrichment methods, which include organic solvent precipitation and ultrafiltration.In this study, we proposed new candidate bioactive peptides by combining this peptide analysis method with functional analyses and the in silico peptide discovery method.
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| Academic Significance and Societal Importance of the Research Achievements |
ペプチドを直接の治療薬として、または創薬シーズとして開発するペプチド創薬への期待は大きいが、新規生理活性ペプチドを効率よく探索する方法論は模索段階にすぎない。近年、オーファンＧタンパク共役型受容体(GPCR)を用いる手法が成果を挙げたが、同定される新規因子は年々減少し、現在も 100 以上の GPCR のリガンドは未同定である。そこで、本研究では、我々の開発した超高感度血中ペプチド探索法を軸に、生理活性ペプチドの新たな探索法を提案し、新規生理活性ペプチドを探索することを目指している。
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Search for a Novel Allergen in Hen's Egg Allergy Using an IgE Immunoblotting Assay.2018
Author(s)
Sogawa K, Takahashi Y, Shibata Y, Satoh M, Kodera Y, Nomura F, Tanaka T, Sato H, Yamaide F, Nakano T, Iwahashi K, Sugita-Konishi Y, Shimada A, Shimojo N.
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Journal Title
Int Arch Allergy Immunol
Volume: 176
Issue: 3-4
Pages: 189-197
DOI
Related Report
Peer Reviewed
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[Journal Article] Assessment by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long-Term Sample Storage.2018
Author(s)
Tsuchida S, Satoh M, Umemura H, Sogawa K, Takiwaki M, Ishige T, Miyabayashi Y, Iwasawa Y, Kobayashi S, Beppu M, Nishimura M, Kodera Y, Matsushita K, Nomura F
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Journal Title
Proteomics. Clinical applications
Volume: 15
Issue: 3
Pages: 47-53
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of LEFTY as a molecular marker for ovarian clear cell carcinoma.2017
Author(s)
Akiya M, Yamazaki M, Matsumoto T, Kawashima Y, Oguri Y, Kajita S, Kijima D, Chiba R, Yokoi A, Takahashi H, Kodera Y, Saegusa M.
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Journal Title
Oncotarget
Volume: 8
Issue: 38
Pages: 63646-63664
DOI
Related Report
Peer Reviewed / Open Access
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