| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
Membrane proteins exert their physiological functions by dynamically changing their localization in living cells. In this study, to visualize the dynamics of endogenous membrane proteins, we developed a hybrid probe consisting of a nanobody complexed with a membrane staining dye using our protein labeling technology. This probe can specifically recognize endogenous membrane proteins and increase their fluorescence intensity, which is a useful technique to detect endogenous membrane proteins with high fluorescence contrast in living cells.
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