| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2021: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Protein methylation, catalyzed by protein methyltransferases (MTases) using S-adenosyl-L-methionine (SAM ) as a methyl donor, is an important post-translational modification. We have focused on the development of a new chemical methylome strategy, using propargylic Se-adenosyl-L-selenomethionine (ProSeAM), an analog of SAM, enabling to identify substrates and methylation sites. Integrating ProSeAM with SILAC (stable isotope labeling by amino acids in cell culture) led us to the development of PMS (ProSeAM/MTase/SILAC) and PIS (ProSeAM/Inhibitor/SILAC), which are useful tools for protein substrate discovery and inhibitor profiling.
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