| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
Using macaques, we conducted the functional neuroimaging during a visual object memory task, and found significant task-related activity in the prefrontal and temporal cortices (PFC and TC). We then injected AAVs into the PFC activation site to express an inhibitory DREADD. The task performance decreased as a result of administration of DCZ, a DREADD agonist we recently developed. The functional neuroimaging during the task with DCZ administration revealed activity decrease in both the DREADD-expressing PFC and the remote TC sites. Finally, we conducted unit recordings in the TC activation site during the task under the silencing of the PFC activation site, and found the corresponding changes in the neuronal activities in the TC site. The combination of DREADD with both the functional neuroimaging and electrophysiology provided a multi-scale causal understanding of the operation and function of a brain-wide network underlying the visual object memory in primates.
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