Multi-scale causal understanding of network operation for object memory in primates
| Project/Area Number |
17H02219
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic / Social brain science
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
Hirabayashi Toshiyuki 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 脳機能イメージング研究部, 主幹研究員(定常) (60376423)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 霊長類 / 化学遺伝学 / 脳機能イメージング / 認知記憶 / 電気生理 / 電気生理学 / 機能イメージング / 記憶 / 視覚記憶 / 多点同時記録 |
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| Outline of Final Research Achievements |
Using macaques, we conducted the functional neuroimaging during a visual object memory task, and found significant task-related activity in the prefrontal and temporal cortices (PFC and TC). We then injected AAVs into the PFC activation site to express an inhibitory DREADD. The task performance decreased as a result of administration of DCZ, a DREADD agonist we recently developed. The functional neuroimaging during the task with DCZ administration revealed activity decrease in both the DREADD-expressing PFC and the remote TC sites. Finally, we conducted unit recordings in the TC activation site during the task under the silencing of the PFC activation site, and found the corresponding changes in the neuronal activities in the TC site. The combination of DREADD with both the functional neuroimaging and electrophysiology provided a multi-scale causal understanding of the operation and function of a brain-wide network underlying the visual object memory in primates.
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| Academic Significance and Societal Importance of the Research Achievements |
認知記憶の障害は、多くの神経・精神疾患における主要な症状の一つである。本研究は、化学遺伝学と認知課題を、機能イメージング、電気生理等のマルチモーダルな神経活動計測と組み合わせる事により、ヒトに近い脳構造と機能を有する霊長類モデルにおいて、認知記憶を支える前頭葉ー側頭葉ネットワークの作動及び機能について、因果的かつマルチスケールな理解をもたらした。これにより、ヒトにおいてそれに対応する症状及びその責任神経回路についての理解が深まり、ひいては新たな治療もしくは介入法等の開発に繋がり得ると考えられる。
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Report
(5 results)
Research Products
(9 results)
