Construction of Vertical Alignment Filters Assembled from Monolayer Membrane Nanochannels and Their Bio-application for Separation and Purification of Proteins

• Project/Area Number: 17H02726
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanostructural chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Kameta Naohiro 国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (20517297)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 自己組織化 / 脂質分子 / 単分子膜 / ナノチャンネル / ナノフィルター / タンパク質 / 分離 / リフォールディング / 分子膜 / 単一分子解析 / 時間分解蛍光顕微鏡 / 蛍光相関分光法 / 有機無機複合化 / ナノ材料 / ゾルゲル反応 / 酵素

Outline of Final Research Achievements

Biotechnologies for separation and purification of proteins have been attracted in the medical fields. Herein we develop hybrid nanofilters based on molecular-monolayer nanochannels that are vertically aligned in a porous anodic alumina. The separation efficiency of proteins strongly depends on the diameters and the surface functional groups of the molecular-monolayer nanochannels. We also succeeded in construction of hybrid nanofilters, which allow us to accelerate the dissociation and the refolding of protein aggregates.

Academic Significance and Societal Importance of the Research Achievements

有用性物質の分離、化学資源の貯蔵、触媒的物質変換、汚染物質の除去、生活水の獲得等の社会ニーズに応えるべく、多種多様な構成成分、次元・形状・サイズを有するフィルターが開発されてきた。フィルター膜に対して口径数十ナノメートル以下の縦穴を整然と集積化できれば、分離理論段数の飛躍的な向上、分離の超高速化だけでなく、フィルターにかかる圧力負荷を大幅に低減可能である。多孔性膜内での自己組織化により形成させる本提案の垂直型ナノフィルターは、目的対象物に応じてその空孔サイズや表面構造をテーラーメイド化できるため、タンパク質の分離精製だけでなく、上記の社会ニーズに対応した技術シーズの創出が期待できる。

Research Products

• [Int'l Joint Research] Kansas State University(米国)
• [Int'l Joint Research] Kansas State University(米国)
• [Int'l Joint Research] Kansas State University(米国)
• [Journal Article] Soft-Matter Nanotubes: A Platform for Diverse Functions and Applications (2020)
  • Author(s): Shimizu Toshimi、Ding Wuxiao、Kameta Naohiro
  • Journal Title: Chemical Reviews Volume: 120 Issue: 4 Pages: 2347-2407
  • DOI: 10.1021/acs.chemrev.9b00509
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Influences of Hydrogen Bonding-Based Stabilization of Bolaamphiphile Layers on Molecular Diffusion within Organic Nanotubes Having Inner Carboxyl Groups (2020)
  • Author(s): Govinda Ghimire、Moore Mikaela M、Leuschen Rebecca、Nagasaka Shinobu、Kameta Naohiro、Masuda Mitsutoshi、Higgins Daniel A、Ito Takashi
  • Journal Title: Langmuir Volume: 36 Issue: 22 Pages: 6145-6153
  • DOI: 10.1021/acs.langmuir.0c00556
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Lipid-assembled Nanotubes for Analytical Chemistry (2019)
  • Author(s): 亀田直弘
  • Journal Title: BUNSEKI KAGAKU Volume: 68 Issue: 9 Pages: 683-697
  • DOI: 10.2116/bunsekikagaku.68.683
  • NAID: 130007723746
  • ISSN: 0525-1931
  • Year and Date: 2019-09-05
  • Peer Reviewed
• [Journal Article] Observing the Kinetic Pathway of Nanotube Formation from Bolaamphiphiles by Time-Resolved Small-Angle X-ray Scattering (2019)
  • Author(s): Takahashi Rintaro、Kawauchi Hiroki、Kameta Naohiro、Lee Ji Ha、Fujii Shota、Shimizu Toshimi、Sakurai Kazuo
  • Journal Title: The Journal of Physical Chemistry B Volume: 123 Issue: 19 Pages: 4340-4345
  • DOI: 10.1021/acs.jpcb.9b01746
  • Peer Reviewed
• [Journal Article] Diffusion Behavior of Differently Charged Molecules in Self-Assembled Organic Nanotubes Studied Using Imaging Fluorescence Correlation Spectroscopy (2019)
  • Author(s): Govinda Ghimire、Roberto Espinoza、Xu Hao、Nagasaka Shinobu、Kameta Naohiro、Masuda Mitsutoshi、Higgins Daniel A、Ito Takashi
  • Journal Title: Langmuir Volume: 35 Issue: 24 Pages: 7783-7790
  • DOI: 10.1021/acs.langmuir.9b01022
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 脂質単分子膜から創るナノチューブ (2019)
  • Author(s): 亀田直弘
  • Journal Title: 高分子 Volume: 68 Pages: 414-415
• [Journal Article] Supramolecular Nanotube Reactors for Production of Imine Polymers with Controlled Conformation, Size, and Chirality (2019)
  • Author(s): Kameta Naohiro、Ding Wuxiao
  • Journal Title: Small Volume: 15 Issue: 19 Pages: 1900682-1900682
  • DOI: 10.1002/smll.201900682
  • Peer Reviewed
• [Journal Article] Direct Joining of a Heterogeneous Pair of Supramolecular Nanotubes and Reaction Control of a Guest Compound by Transportation in The Nanochannels (2019)
  • Author(s): Kameta Naohiro、Ding Wuxiao
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 92 Issue: 6 Pages: 1053-1059
  • DOI: 10.1246/bcsj.20190046
  • NAID: 130007663604
  • Peer Reviewed
• [Journal Article] Encapsulation of Albumin in Organic Nanotube Channel: Structural Investigation by Small-Angle X-ray Scattering (2019)
  • Author(s): Kawauchi Hiroki、Takahashi Rintaro、Kameta Naohiro、Fujii Shota、Lee Ji Ha、Shimizu Toshimi、Sakurai Kazuo
  • Journal Title: ACS Applied Bio Materials Volume: 2 Issue: 4 Pages: 1652-1659
  • DOI: 10.1021/acsabm.9b00047
  • Peer Reviewed
• [Journal Article] PEG-nanotube liquid crystals as templates for construction of surfactant-free gold nanorods (2018)
  • Author(s): Kameta Naohiro、Shiroishi Hidenobu
  • Journal Title: Chemical Communications Volume: 54 Issue: 37 Pages: 4665-4668
  • DOI: 10.1039/c8cc02013b
  • Peer Reviewed
• [Journal Article] Shrinkable Nanotubes for Duplex Formation of Short Nucleotides (2018)
  • Author(s): Kameta Naohiro、Akiyama Haruhisa
  • Journal Title: Small Volume: 14 Issue: 34 Pages: 1801967-1801967
  • DOI: 10.1002/smll.201801967
  • Peer Reviewed
• [Journal Article] Bioreactors Based on Enzymes Encapsulated in Photoresponsive Transformable Nanotubes and Nanocoils End-Capped with Magnetic Nanoparticles (2018)
  • Author(s): Kameta Naohiro、Manaka Yuichi、Akiyama Haruhisa、Shimizu Toshimi
  • Journal Title: Advanced Biosystems Volume: 2 Issue: 4 Pages: 1700214-1700214
  • DOI: 10.1002/adbi.201700214
  • Peer Reviewed
• [Journal Article] Thermoresponsive PEG-Coated Nanotubes as Chiral Selectors of Amino Acids and Peptides (2018)
  • Author(s): Kameta Naohiro、Dong Jiuchao、Yui Hiroharu
  • Journal Title: Small Volume: 14 Issue: 15 Pages: 1800030-1800030
  • DOI: 10.1002/smll.201800030
  • Peer Reviewed
• [Journal Article] Soft Nanotubes Derivatized with Short PEG Chains for Thermally Controllable Extraction and Separation of Peptides (2017)
  • Author(s): Kameta Naohiro、Ding Wuxiao、Dong Jiuchao
  • Journal Title: ACS Omega Volume: 2 Issue: 9 Pages: 6143-6150
  • DOI: 10.1021/acsomega.7b00838
  • Peer Reviewed / Open Access
• [Journal Article] Enhancement of the photocatalytic activity of rhenium(i) complexes by encapsulation in light-harvesting soft nanotubes (2017)
  • Author(s): Kameta Naohiro、Aoyagi Masaru、Asakawa Masumi
  • Journal Title: Chem. Commun. Volume: 53 Issue: 73 Pages: 10116-10119
  • DOI: 10.1039/c7cc05337a
  • Peer Reviewed
• [Journal Article] ナノチューブゲルの創製とバイオ・グリーン応用 (2017)
  • Author(s): 亀田直弘
  • Journal Title: オレオサイエンス Volume: 17 Pages: 623-631
  • NAID: 130007687610
• [Journal Article] Spectroscopic imaging studies of nanoscale polarity and mass transport phenomena in self-assembled organic nanotubes (2017)
  • Author(s): Xu Hao、Nagasaka Shinobu、Kameta Naohiro、Masuda Mitsutoshi、Ito Takashi、Higgins Daniel A.
  • Journal Title: Physical Chemistry Chemical Physics Volume: 19 Issue: 30 Pages: 20040-20048
  • DOI: 10.1039/c7cp03672h
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cross-sectional structures of a molecular monolayer nanotube explored with SAXS: evidence for the parallel orientation of the headgroups in asymmetric bolaamphiphiles (2017)
  • Author(s): Yoshida Kenta、Takahashi Rintaro、Fujii Shota、Kameta Naohiro、Shimizu Toshimi、Sakurai Kazuo
  • Journal Title: Physical Chemistry Chemical Physics Volume: 19 Issue: 36 Pages: 24445-24447
  • DOI: 10.1039/c7cp03905k
  • Peer Reviewed
• [Presentation] 超分子ナノチューブ液晶を鋳型に用いた界面活性剤フリー金ナノロッドの構築とイオン・キラル認識 (2019)
  • Author(s): 亀田直弘、丁武孝
  • Organizer: 第１７回ホスト－ゲスト・超分子化学シンポジウム
• [Presentation] 光刺激形態可変性ナノチューブ・ナノコイル：酵素の包接化及び磁性ナノ粒子でキャッピングしたバイオリアクター (2019)
  • Author(s): 亀田直弘、眞中雄一、秋山陽久
  • Organizer: 第６８回高分子学会年次大会
• [Presentation] Monolayer-Based Nanotubes with Controllable Diameters (2019)
  • Author(s): Kameta Naohiro、Ding Wuxiao
  • Organizer: Okinawa Colloids 2019
  • Int'l Joint Research
• [Presentation] 光刺激形態可変機能を持つナノチューブ・ナノコイル ～酵素の包接化及び磁性ナノ粒子でキャッピングしたバイオリアクター～ (2018)
  • Author(s): 亀田直弘、眞中雄一、秋山陽久
  • Organizer: 第１６回ホスト－ゲスト・超分子化学シンポジウム
• [Presentation] ナノチューブ液晶を鋳型に用いた界面活性剤フリー金ナノロッドの構築とイオン・キラルセンシング (2018)
  • Author(s): 亀田直弘、丁武孝
  • Organizer: 日本分析化学会第６７年会
• [Presentation] 酵素固定化担体としての光刺激形態可変性ナノチューブ・ナノコイル (2018)
  • Author(s): 亀田直弘、秋山陽久
  • Organizer: 化学とマイクロ・ナノシステム学会第38回研究会
• [Presentation] 分析化学への貢献を目指したソフトナノチューブの開発 (2018)
  • Author(s): 亀田直弘
  • Organizer: 第15回茨城地区分析技術交流会
  • Invited
• [Presentation] 中分子や高分子を安定に貯蔵できるナノカプセルを開発 (2018)
  • Author(s): 亀田直弘
  • Organizer: サステナブル技術連携促進シンポジウム「ヘルスケア」
• [Presentation] Diffusion behavior of charged dye molecules in self-assembled organic nanotubes studied using imaging fluorescence correlation spectroscopy (2018)
  • Author(s): Ghimire Govinda、Espinoza Roberto、Hao Xu、Shinobu Nagasaka、亀田直弘、増田光俊、Daniel Higgins、Takashi Ito
  • Organizer: 256th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nanoscience, Nanotechnology and Beyond
  • Int'l Joint Research
• [Presentation] 短鎖PEG修飾ソフトナノチューブのタンパク質凝集抑制効果 (2017)
  • Author(s): 亀田直弘、松沢智彦、矢追克郎
  • Organizer: 第６６回高分子討論会
• [Presentation] 糖脂質から成るナノチューブ・ベシクルの熱相転移を利用したタンパク質凝集体の可溶化とリフォールディング促進 (2017)
  • Author(s): 亀田直弘、松沢智彦、矢追克郎
  • Organizer: 第６８回コロイドおよび界面化学討論会
• [Presentation] Soft Nanotube Materials for Biological Applications (2017)
  • Author(s): Naohiro Kameta
  • Organizer: 5th Nano Today Conference
  • Int'l Joint Research / Invited
• [Remarks] https://unit.aist.go.jp/nmri/index.html
• [Remarks] 産業技術総合研究所ナノ材料研究部門
  • URL: https://unit.aist.go.jp/nmri/index.html