Efficient fluorescence detection of single molecules in liquid by using optical nanofibers
| Project/Area Number |
17H02741
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials chemistry
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| Research Institution | Osaka City University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | ナノデバイス / 光ファイバ / ナノ粒子 / 化学分析 / ナノ光ファイバ / 生体計測 / センシング / 蛍光ナノ粒子 / マイクロ・ナノデバイス |
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| Outline of Final Research Achievements |
In nanophotonic devices, substantial enhancement of optical field is realized by confining photons in a small area, which enables strong light-matter interaction. In this research, we study optical nanofibers that provide efficient optical coupling between the fluorescent two level systems (molecules, nanoparticles, etc) and optical fiber mode for the use in aqueous media. The main results are as follows: (1) A single-ended optical nanofibers were fabricated and applied to the nematode C. elegans. (2) The surface state and optical (and spin) properties of gold or diamond nanoparticles were quantified for their better integration with nanofibers. In addition, pH dependence of nanodiamond spin properties was determined. (3) Temperature sensing of cells and nematodes was realized using fluorescent nanodiamonds.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、量子エレクトロニクス分野で開発してきたナノ光ファイバデバイスを水溶液中での利用に耐えうるものとしようとするものである。このナノ光ファイバは空気中などでは超高効率蛍光検出が可能であり、その詳細な動作特性も明らかとなっていたが、本研究により、ナノ光ファイバの溶液中利用における動作特性の理解が進展した。また、細胞や線虫と言った実際的な生体試料に適用した際の利用例を、細胞温度測定という観点から部分的に示すことができた。将来的には、生体内での温度モニタリングなどの技術にもつながると期待される。
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Report
(4 results)
Research Products
(62 results)
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[Journal Article] Fiber-Coupled Diamond Micro-Waveguides toward an Efficient Quantum Interface for Spin Defect Centers2017
Author(s)
Masazumi Fujiwara, Oliver Neitzke, Tim Schroder, Andreas W. Schell, Janik Wolters, Jiabao Zheng, Sara Mouradian, Mohamed Almoktar, Shigeki Takeuchi, Dirk Englund, and Oliver Benson
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Journal Title
ACS OMEGA
Volume: 2
Issue: 10
Pages: 7194-7202
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Realtime nanodiamond thermometry probing in-vivo thermogenic responses2019
Author(s)
M. Fujiwara, S. Sun, A. Dohms, Y. Nishimura, K. Suto, Y. Takezawa, K. Oshimi, L. Zhao, N. Sadzak, Y. Umehara, Y. Teki, N. Komatsu, O. Benson, Y. Shikano, E. Kage-Nakadai
Organizer
International Workshop on Quantum Sensing & Biophotonics 2019
Related Report
Int'l Joint Research
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