| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
This study aimed to construct a three-dimensional culture model that mimics the tissues of the posterior segment and the anterior segment of the eye using micro/nano technology. Regarding the posterior segment tissues, as a model of the outer blood-retinal barrier (BRB), the retinal pigment epithelial cell layer and the artificial choroid were integrated into a two-layered microfluidic chip, where both tissues were able to interact. Also, as a model of the inner BRB, a sprouting system of retinal microvessels was investigated using a microfluidic chip. Regarding the anterior segment tissues, an artificial eyelid that can be integrated into a corneal culture model was developed. A linear actuator was used to enable horizontal movement simulating the blinking of healthy subjects and dry eye patients, and the movement of the artificial eyelid was confirmed using isolated pig eyeballs.
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