| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
In this study, we have developed a technique for isolating chromosomes from mammalian single cells under a microscope in a microfluidic device, unfolding them, and stretching them in a gentle flow. By applying this technique to mouse-derived undifferentiated cells (ES cells) and differentiated cells (fibroblasts), we found that undifferentiated cell-derived chromosomes were easier to unfold. In addition, for the purpose of determining chromosome numbers, we worked on visualization of specific DNA sequences on the chromosomes in the microfluidic device using the fluorescence-labeled dCas9 protein as a probe. Depending on the target sequence selected, the visualization was achieved.
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