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Graphene bio-interface created on inner wall of micro-roll

Research Project

Project/Area Number 17H02759
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nano/Microsystems
Research InstitutionNTT Basic Research Laboratories

Principal Investigator

Ueno Yuko  日本電信電話株式会社NTT物性科学基礎研究所, 機能物質科学研究部, 特別研究員 (30589627)

Co-Investigator(Kenkyū-buntansha) 手島 哲彦  日本電信電話株式会社NTT物性科学基礎研究所, 機能物質科学研究部, 研究員 (90779183)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywordsグラフェン / アプタマ / バイオセンサ / 蛍光 / 三次元 / 生体適合材料 / DNA / 細胞分泌物 / アポトーシス / マイクロ・ナノデバイス / 生体材料 / ナノ材料 / 構造・機能材料 / 分子認識 / 自己組み立て / 生体分子 / 3次元構造
Outline of Final Research Achievements

We have established a method for producing tubular microenvelopes with high yield by using a laminated film composed of highly biocompatible polymer layers of reduced graphene oxide (rGO) / polyparaxylene / graphene / alginate gel. A three-dimensional graphene aptasensor was prepared by modifying the DNA aptamer that recognizes cytochrome C, a protein related to apoptosis, on the surface of rGO on the inner wall. We succeeded in spatiotemporally detecting the release of cytochrome C during apoptosis by artificially inducing apoptosis by encapsulating cells alive in the aptasensor. Regarding these achievements, we made presentations at academic conferences and papers at home and abroad, including invited lectures.

Academic Significance and Societal Importance of the Research Achievements

分子や細胞を内包可能な筒状マイクロエンベロープの内壁に,分子認識場として動作するグラフェンバイオセンサを構築し,内部の中空空間に閉じ込められた生体分子を高感度にかつ選択的に検出する新規プラットフォームを確立し,生きた細胞から放出される分泌物の検出デモンストレーションに成功した.本研究の成果として,従来は物質の拡散により単利同定が困難であった,細胞の外部環境と放出される分泌物の関連解明など,細胞活動の研究に展開可能な新規な可視化技術を提供し,新たな学術分野研究テーマを開拓した.

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (36 results)

All 2020 2019 2018 2017

All Journal Article (9 results) (of which Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (25 results) (of which Int'l Joint Research: 14 results,  Invited: 10 results) Book (2 results)

  • [Journal Article] 分子認識機能材料の創生とマイクロ分析への応用2020

    • Author(s)
      上野 祐子
    • Journal Title

      分析化学

      Volume: -

    • NAID

      130007937257

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adhesive Layer for Robust Graphene Transferred on Solid Support and Its Application to Graphene Microelectrode Manufacturing2019

    • Author(s)
      Ueno Yuko、Dendo Kenji、Homma Yukihide、Furukawa Kazuaki
    • Journal Title

      Sensors and Materials

      Volume: 31 Issue: 4 Pages: 1157

    • DOI

      10.18494/SAM.2019.2237

    • ISSN
      0914-4935, 2435-0869
    • Year and Date
      2019-04-11
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Graphene-based neuron encapsulation with controlled axonal outgrowth2019

    • Author(s)
      Sakai Koji、Teshima Tetsuhiko F.、Nakashima Hiroshi、Ueno Yuko
    • Journal Title

      Nanoscale

      Volume: 11 Issue: 28 Pages: 13249-13259

    • DOI

      10.1039/c9nr04165f

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Self-Folded Three-Dimensional Graphene with a Tunable Shape and Conductivity2019

    • Author(s)
      Teshima Tetsuhiko F.、Henderson Calum S.、Takamura Makoto、Ogawa Yui、Wang Shengnan、Kashimura Yoshiaki、Sasaki Satoshi、Goto Toichiro、Nakashima Hiroshi、Ueno Yuko
    • Journal Title

      Nano Letters

      Volume: 19 Issue: 1 Pages: 461-470

    • DOI

      10.1021/acs.nanolett.8b04279

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 薄膜の三次元自己組立てを利用 した細胞の立体形成技術2019

    • Author(s)
      手島哲彦
    • Journal Title

      PHARMSTAGE 《シリーズiPS 細胞・再生医療》

      Volume: 18 Pages: 1-5

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Fabrication of Graphene Microroll Aptasensor2018

    • Author(s)
      Ueno Yuko、Teshima Tetsuhiko、Henderson Calum、Nakashima Hiroshi
    • Journal Title

      Sensors and Materials

      Volume: 30 Issue: 12 Pages: 2989

    • DOI

      10.18494/SAM.2018.2069

    • ISSN
      0914-4935, 2435-0869
    • Year and Date
      2018-12-28
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] On-Chip FRET Graphene Aptasensor2018

    • Author(s)
      Y. Ueno K. Furukawa
    • Journal Title

      International Journal of Automation Technology

      Volume: 12 Issue: 1 Pages: 37-44

    • DOI

      10.20965/ijat.2018.p0037

    • NAID

      130007505910

    • ISSN
      1881-7629, 1883-8022
    • Year and Date
      2018-01-05
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 高分子積層薄膜を用いた細胞の内包化と三次元自己組立て技術2018

    • Author(s)
      手島哲彦, 中島寛, 上野祐子, 佐々木智, ヘンダーソン カルム, 塚田信吾
    • Journal Title

      化学とマイクロ・ナノシステム

      Volume: 17 Pages: 35-38

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Cell encapsulation and 3D self-assembly using multi-layered polymeric thin films2018

    • Author(s)
      T. Teshima, H. Nakashima, Y. Ueno, S. Sasaki, C. S. Henderson, S. Tsukada
    • Journal Title

      NTT Technical Review

      Volume: 16 Pages: 53-61

    • NAID

      40021536762

    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Presentation] Graphene-based Micro Biosensors2019

    • Author(s)
      Y. Ueno
    • Organizer
      Matrafured International Conference on Chemical Sensors
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Graphene-based Micro Biosensors2019

    • Author(s)
      Y. Ueno
    • Organizer
      The Seventeenth International Symposium on Electroanalytical Chemistry & The Third International Meeting on Electrogenerated Chemiluminescence
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Graphene-mediated micro-patterning of conductive polymers toward implantable electrodes2019

    • Author(s)
      T. Teshima, K. Sakai, Y. Kashimura, H. Miyazako, H. Nakashima, S. Tsukada, Y. Ueno, T. Osaki, S. Takeuchi
    • Organizer
      MicroTAS 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Graphene-based Micro Biosensors2019

    • Author(s)
      Y. Ueno
    • Organizer
      Okinawa Colloids 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Graphene-based Micro Biosensors2019

    • Author(s)
      Y. Ueno
    • Organizer
      Asian Conference on Chemical Sensors
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Spatiotemporal Detection of Cytoplasmic Proteins by Three-dimensional Graphene-oxide Aptamer Sensor2019

    • Author(s)
      H. Miyazako, T. Teshima, Y. Ueno
    • Organizer
      International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS & Applications
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 三次元神経回路網構築のための薄膜組み立て技術を用いた神経細胞塊形成2019

    • Author(s)
      酒井洸児,手島 哲彦,中島 寛,上野 祐子
    • Organizer
      電気学会 電子・情報・システム部門大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] グラフェンFRETアプタセンサにおける蛍光強度の層数依存性2019

    • Author(s)
      上野祐子,宮廻裕樹,古川一暁
    • Organizer
      電気化学秋季大会(化学センサ研究会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 分子認識機能材料の創生とマイクロ分析への応用2019

    • Author(s)
      上野祐子
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] グラフェン表面を用いた蛍光検出型マイクロバイオセンサ2019

    • Author(s)
      上野祐子
    • Organizer
      マイクロビームアナリシス研究会(学振141研究会)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] グラフェンFRETアプタセンサにおけるグラフェン層数による蛍光強度変化2019

    • Author(s)
      上野祐子,宮廻裕樹,古川一暁
    • Organizer
      電子情報通信学会 有機エレクトロニクス研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 酸化グラフェンDNA アプタマ センサ を用いた細胞分泌物の時空間計測2019

    • Author(s)
      宮廻裕樹,手島哲彦, 上野祐子
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Graphene-based Micro Biosensors2018

    • Author(s)
      Ueno Yuko
    • Organizer
      2018 China-Japan-Korea Symposium on Analytical Chemistry
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Graphene-driven 3D self-assembly of conductive polymeric micro-rolls2018

    • Author(s)
      T. F. Teshima, C. S. Henderson, M. Takamura, Y. Ogawa, S. Wang, Y. Kashimura, S. Sasaki, T. Goto, H. Nakashima, Y. Ueno
    • Organizer
      Biointerfaces Inter., 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Graphene Micro-roll Aptasensor for Three-Dimensional Protein Detection2018

    • Author(s)
      Y. Ueno, T. Teshima, C. Henderson, H. Nakashima
    • Organizer
      International Meeting on Chemical Sensors
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Graphene Aptasensor Built at the Inner Wall of a Hollow 3D Structure2018

    • Author(s)
      Y. Ueno, T. Teshima, C. Henderson, H. Nakashima
    • Organizer
      10th International Symposium on Organic Molecular Electronics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neuron-encapsulated self-foldable graphene for graft-electrode interface2018

    • Author(s)
      Koji Sakai, Tetsuhiko Teshima, Hiroshi Nakashima, Yuko Ueno
    • Organizer
      2018 MRS Fall Meeting & Exhibit
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 単層グラフェンの転写による高分子薄膜の三次元自己組立て2018

    • Author(s)
      手島哲彦,Calum S. Henderson,高村真琴,小川友以,Shengnan Wang,樫村吉晃,佐々木智,後藤東一郎,酒井洸児,中島寛,上野祐子
    • Organizer
      化学とマイクロ・ナノシステム第38回研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ホールアレイ筒状グラフェンを用いた三次元培養神経組織の形成2018

    • Author(s)
      酒井洸児,手島哲彦,中島寛,上野祐子
    • Organizer
      化学とマイクロ・ナノシステム第38回研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 円筒型グラフェンアプタセンサを用いた3次元タンパク質検出2018

    • Author(s)
      上野祐子,手島哲彦,Calum Henderson,中島寛
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Graphene FRET aptasensor built on a solid support2017

    • Author(s)
      Y. Ueno, T. Teshima, C. Henderson, H. Nakashima
    • Organizer
      253rd American Chemical Society National Meeting & Exposition
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Graphene-based Aptasensors on Self-folded Substrates2017

    • Author(s)
      C. Henderson, Y. Ueno, T. Teshima, H. Nakashima
    • Organizer
      RSC Tokyo International Conference, JASIS Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Three-Dimensional Protein Detection by Graphene Micro-roll Aptasensor2017

    • Author(s)
      Y. Ueno, T. Teshima, C. Henderson, H. Nakashima
    • Organizer
      International Conference on BioSensors, BioElectronics, Biomedical Devices, BioMEMS/NEMS & Applications
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] グラフェンとDNAのハイブリッド:タンパク質検出センサの構築2017

    • Author(s)
      古川一暁,上野祐子
    • Organizer
      2017年電子情報通信学会ソサイエティ大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ベシクル内部から放出された化学物質のグラフェンアプタセンサによる検出2017

    • Author(s)
      上野祐子, 古川一暁, 樫村吉晃
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Book] Chemical, Gas, and Biosensors for Internet of Things and Related Applications2019

    • Author(s)
      Kohji Mitsubayashi Osamu Niwa Yuko Ueno (Editor)
    • Total Pages
      406
    • Publisher
      Elsevier
    • ISBN
      9780128154090
    • Related Report
      2019 Annual Research Report
  • [Book] Chemical, Gas, and Biosensors for Internet of Things and Related Applications2019

    • Author(s)
      R. Kurita, O. Niwa, Y. Ueno
    • Total Pages
      416
    • Publisher
      Elsevier
    • ISBN
      0128154098
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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