• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

High-speed and high-resolution Raman microscopy using patterned illumination

Research Project

Project/Area Number 17H02800
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Optical engineering, Photon science
Research InstitutionOsaka University

Principal Investigator

Fujita Katsumasa  大阪大学, 工学研究科, 教授 (80362664)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2017: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsラマン顕微鏡 / ラマン分光法 / 超解像顕微鏡 / 分光分析 / 無標識観察 / ラマン散乱 / 構造化照明顕微鏡 / 高速イメージング / ラベルフリーイメージング / ラマンタグ / ラマン分光 / 構造化照明 / ラマン散乱顕微鏡 / 顕微鏡光学
Outline of Final Research Achievements

In this research, we aimed to develop a novel Raman microscopy technique that can achieve high-speed and high-resolution imaging by using programmable patterned illumination. Using a spatial light modulator, a light illumination pattern on a sample was controlled so that one can choose an illumination mode suitable for high-speed imaging and/or high-resolution imaging. Several types of lattice illuminations have been tested and demonstrated up to two folds and 20 folds improvements of the spatial and temporal resolution, respectively. We have also developed optical systems that can detect two different wavenumber regions simultaneously to enable more chemical information from samples.

Academic Significance and Societal Importance of the Research Achievements

ラマン散乱顕微鏡は、試料内の分子や結晶構造を分析しながら、それらの空間分布を観察する技術として活用されている。しかしながら、その空間分解能、および時間分解能はラマン散乱の効率の低さに大きく制限され、多くの応用においてその分析力を生かせない状態にある。本研究では、微弱なラマン散乱を用いても、高速かつ高空間分解に各種試料の顕微鏡観察を可能とする、新規の光学技術開発の開発に成功した。また、応用研究においては、開発した技術が、高分子材料、生体試料の観察に実際に適用可能であること、細胞内に分布した高分子材料とを分光分析により見極められること、また細胞種の分別が可能であること示した。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (47 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (17 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (26 results) (of which Int'l Joint Research: 14 results,  Invited: 26 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] コロンビア大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Leibniz Institute of Photonic Technology(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Direct visualization of an antidepressant analog using surface-enhanced Raman scattering in the brain2020

    • Author(s)
      Masato Tanuma, Atsushi Kasai, Kazuki Bando, Naoyuki Kotoku, Kazuo Harada, Masafumi Minoshima, Kosuke Higashino, Atsushi Kimishima, Masayoshi Arai, Yukio Ago, Kaoru Seiriki, Kazuya Kikuchi, Satoshi Kawata, Katsumasa Fujita, Hitoshi Hashimoto
    • Journal Title

      JCI Insight

      Volume: 5 Issue: 6

    • DOI

      10.1172/jci.insight.133348

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alkyne-tag SERS imaging for visualizing small molecule drugs in live cells2020

    • Author(s)
      Koike Kota、Bando Kazuki、Ando Jun、Smith Nicholas I.、Dodo Kosuke、Kawata Satoshi、Sodeoka Mikiko、Fujita Katsumasa
    • Journal Title

      Proc. SPIE

      Volume: 11219 Pages: 12-12

    • DOI

      10.1117/12.2545872

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 超解像バイオイメージング2020

    • Author(s)
      藤田克昌
    • Journal Title

      光技術コンタクト

      Volume: 58 Pages: 3-9

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging2019

    • Author(s)
      Ryosuke Oketani, Haruka Suda, Kumiko Uegaki, Toshiki Kubo, Tomoki Matsuda, Masahito Yamanaka, Yoshiyuki Arai, Nicholas I. Smith, Takeharu Nagai, Katsumasa Fujita
    • Journal Title

      JOURNAL OF BIOMEDICAL OPTICS

      Volume: 25 Issue: 01 Pages: 014502-014502

    • DOI

      10.1117/1.jbo.25.1.014502

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Raman spectroscopic histology using machine learning for nonalcoholic fatty liver disease2019

    • Author(s)
      Khalifa Mohammad Helal, James Nicholas Taylor, Harsono Cahyadi, Akira Okajima, Koji Tabata, Yoshito Itoh, Hideo Tanaka, Katsumasa Fujita, Yoshinori Harada, Tamiki Komatsuzaki
    • Journal Title

      FEBS Letters

      Volume: 593 Issue: 18 Pages: 2535-2544

    • DOI

      10.1002/1873-3468.13520

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High-Resolution Raman Microscopic Detection of Follicular Thyroid Cancer Cells with Unsupervised Machine Learning2019

    • Author(s)
      J. Nicholas Taylor, Kentaro Mochizuki, Kosuke Hashimoto, Yasuaki Kumamoto, Yoshinori Harada, Katsumasa Fujita, Tamiki Komatsuzaki
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 123 Issue: 20 Pages: 4358-4372

    • DOI

      10.1021/acs.jpcb.9b01159

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ラマン散乱顕微鏡が拓く新しいバイオイメージング2019

    • Author(s)
      望月健太郎, 藤田克昌
    • Journal Title

      生産と技術

      Volume: 71 Pages: 28-32

    • NAID

      40021887295

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 非線形な光学応答を利用した超解像顕微鏡2019

    • Author(s)
      藤田克昌
    • Journal Title

      光アライアンス

      Volume: 30 Pages: 6-10

    • Related Report
      2019 Annual Research Report
  • [Journal Article] High-Throughput Cell Imaging and Classification by Narrowband and Low-Spectral-Resolution Raman Microscopy2019

    • Author(s)
      Kumamoto Yasuaki、Mochizuki Kentaro、Hashimoto Kosuke、Harada Yoshinori、Tanaka Hideo、Fujita Katsumasa
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 123 Issue: 12 Pages: 2654-2661

    • DOI

      10.1021/acs.jpcb.8b11295

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction.2019

    • Author(s)
      Morimoto T, Chiu LD, Kanda H, Kawagoe H, Ozawa T, Nakamura M, Nishida K, Fujita K, Fujikado T
    • Journal Title

      Analyst

      Volume: 144 Issue: 8 Pages: 25312540-25312540

    • DOI

      10.1039/c8an02213e

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cell type discrimination based on image features of molecular component distribution2018

    • Author(s)
      A. Germond, T. Ichimura, L.-d. Chiu, K. Fujita, T. M. Watanabe, H. Fujita
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 11726-11726

    • DOI

      10.1038/s41598-018-30276-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ラマン顕微鏡2018

    • Author(s)
      望月健太郎, 藤田克昌
    • Journal Title

      実験医学

      Volume: 36 Pages: 68-70

    • Related Report
      2018 Annual Research Report
  • [Journal Article] ラマン散乱顕微鏡による分析イメージング ~新しい顕微分析技術:カーボン材料から生体試料まで~2018

    • Author(s)
      藤田克昌, 太田泰輔
    • Journal Title

      電気評論

      Volume: 103 Pages: 24-29

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 超解像顕微鏡の原理と展望2018

    • Author(s)
      藤田克昌
    • Journal Title

      応用物理

      Volume: 87 Pages: 164-170

    • NAID

      130007715725

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 構造化照明ラマン顕微鏡によるバイオイメージング2018

    • Author(s)
      渡辺 梢、藤田克昌
    • Journal Title

      オプトロニクス

      Volume: 38 Pages: 83-87

    • Related Report
      2017 Annual Research Report
  • [Journal Article] ラマン散乱顕微鏡を用いた生細胞イメージング2017

    • Author(s)
      藤田克昌
    • Journal Title

      生体の科学

      Volume: 68 Pages: 396-397

    • Related Report
      2017 Annual Research Report
  • [Journal Article] ラマン分光顕微鏡とライフサイエンス応用2017

    • Author(s)
      安藤 潤, 藤田 克昌
    • Journal Title

      光アライアンス

      Volume: 28 Pages: 52-57

    • Related Report
      2017 Annual Research Report
  • [Presentation] Raman microscopy using a multi-slit spectrophotometer for high throughput cell analysis2020

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SPIE Photonics West
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Detecting drugs in live cells by SERS microscopy2020

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SPIE Photonics West
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visible-wavelength two-photon excitation for high-resolution microscopy and microfabrication2020

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SPIE Photonics West
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ラマン散乱を利用した顕微イメージング技術の開発2020

    • Author(s)
      藤田克昌
    • Organizer
      大阪工研協会 第44回分析展と講演・技術発表会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 金属ナノ粒子を用いた細胞内分子の表面増強ラマンイメージング2020

    • Author(s)
      藤田克昌
    • Organizer
      電子情報通信学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ハイスループットなラマン分光分析およびイメージング:並列検出分光光学系の利用2019

    • Author(s)
      藤田克昌
    • Organizer
      第17回 医用分光学研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Raman microscopy for high-throughput molecular analysis,2019

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SCIX2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Label-free cell analysis by Raman microscopy2019

    • Author(s)
      Katsumasa Fujita
    • Organizer
      第78回日本癌学会学術総会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] High-speed, high-resolution Raman microscopy for molecular imaging of biological samples2019

    • Author(s)
      Katsumasa Fujita
    • Organizer
      ICAVS10
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 超解像顕微鏡:原理と応用2019

    • Author(s)
      藤田克昌
    • Organizer
      第16回プラズモニクスシンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ラマン散乱顕微鏡の高速化と高解像度化2019

    • Author(s)
      藤田克昌
    • Organizer
      日本学術振興会 ナノプローブテクノロジー第167委員会 第91回研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ハイスループット細胞分析に向けたラマン分光イメージング技術の開発2019

    • Author(s)
      望月健太郎, 藤田克昌
    • Organizer
      分光学会 生細胞分光部会研究会シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Raman microscopy for analysis of intracellular molecules2019

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Telluride Workshop
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Raman microscopy for analytical imaging of living cells2019

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SPIE Photonics West
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ラマン顕微イメージングによる小分子検出2019

    • Author(s)
      藤田克昌
    • Organizer
      レーザー学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Alkyne-tag Raman imaging for observation of small molecules in living cells2018

    • Author(s)
      Katsumasa Fujita
    • Organizer
      APC2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Large-area Raman imaging by multiple slit detection2018

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SCIX2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Surpassing the diffraction limit in Raman microscopy2018

    • Author(s)
      Katsumasa Fujita
    • Organizer
      ICTON2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 構造化照明顕微鏡による高解像ラマンイメージング2018

    • Author(s)
      藤田克昌
    • Organizer
      光設計研究グループ第64回研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 超解像バイオイメージングの原理と応用,2017

    • Author(s)
      藤田克昌
    • Organizer
      第60回光波センシング技術研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 無標識細胞分析:ラマン分光イメージングの活用2017

    • Author(s)
      藤田克昌
    • Organizer
      関西再生医療産業コンソーシアム 第3回検討会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] The taming of the SERS2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SCIX 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High speed Raman imaging for chemical profiling of cells and tissues,2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      生物物理学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Raman microscopy for molecular imaging of biological samples2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      ICAVS-9
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Raman microscopy for molecular imaging of living cells2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      5th International Symposium on Bioimaging
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Improvement of spatial and spectral resolution in Raman microscopy,2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Biomedical Imaging and Sensing Conference (BISC) 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Label-Free Super-Resolution Microscopy2019

    • Author(s)
      Katsumasa Fujita
    • Total Pages
      498
    • Publisher
      Springer Nature
    • ISBN
      9783030217228
    • Related Report
      2019 Annual Research Report
  • [Remarks] Katsumasa Fujita, Osaka University

    • URL

      http://lasie.ap.eng.osaka-u.ac.jp/ap1g1kat/index_j.html

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi