| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Various hierarchical chiral structures exist from subnano- to micro-meter scales in biological systems. Observation of fluorescence or optical extinction is routinely made for tracking positions of target molecules. In addition, optical activity signals due to chirality of the molecules/molecular assemblies may provide invaluable information to reveal biological functions and origin of asymmetric morphology. Circular dichroism (CD) microscopy enables direct visualization of the spatial CD signal distribution. However, it has not been commonly employed, because artifacts arising from linear dichroism (LD) of the sample interfere in the CD signal obtained by the conventional CD detection method. Recently, we proposed a new polarization modulation for accurate CD detection, which greatly suppressed the LD interference and achieved reliable CD imaging. Based on this, we demonstrated analysis of individual chiral particles and also show possibility of the reflection CD imaging analysis etc.
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