| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
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| Outline of Final Research Achievements |
Operating the analytical column at a high temperature increases the efficiency and speed of the liquid chromatography (LC) due to the reduced viscosity and an increase of solute diffusion speed. However, the maximum temperature of the conventional method is limited by the boiling of the mobile phase to ~90 C. We have developed in this project a novel analytical system that combined the high temperature-LC (HT-LC) with mass spectrometry (MS) via high-pressure electrospray ionization (HP-ESI). The direct coupling of HP-ESI to HT-LC operated at > 100 C was made possible by using the ion source as the pressure regulator. For a given solution flow rate, the analytical time was significantly shortened by increasing the LC column to 160 C. HT-LC-ESI-MS using pure water as the mobile phase with a capillary column was also found to be possible. Carbon and polymer columns were found to be suitable for the HT-LC-ESI-MS of pharmaceuticals and biomolecules such as peptides and proteins.
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