| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
The method of manipulating genomic DNA is useful as a fundamental therapy for genetic diseases. In this study, we demonstrated a photochemical genomic DNA manipulation using ultrafast DNA photo-cross-linking. The photo-cross-linkable probes containing 3-cyanovinylcarbazole and cyanouridine or C3 spacer as inhibitor of photo-cross-linking between probes can photo-cross-link to duplex DNA. In addition, the double stranded DNA photo-cross-linked with probe DNA was inhibited transcription by T7 RNA polymerase and the inhibition of gene expression in cells was confirmed.
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