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Real-Time Single-Molecule Observation of DNA Conformational Cahnges

Research Project

Project/Area Number 17H03088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bio-related chemistry
Research InstitutionOsaka University

Principal Investigator

KAWAI KIYOHIKO  大阪大学, 産業科学研究所, 准教授 (50314422)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
KeywordsDNA / 高次構造 / 1分子検出 / 構造転移 / 速度 / 蛍光 / blinking / 1分子計測 / 1分子計測 / DNA高次構造 / 蛍光相関分光
Outline of Final Research Achievements

We have developed a single-molecule analysis and diagnosis method based on the understanding, control, and utilization of fluorescence blinking (KACB method).
By controlling the blinking by oxidation/reduction reactions, we achieved single molecule discrimination of DNA hairpin, double-stranded, B-type, and A-type structures, and developed a new method for single molecule detection of DNA.
By tracking the temporal change of blinking pattern, we succeeded in single molecule detection of structural transition of functional RNA.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した、KACB法は、様々な分子の1分子検出、そして、その分子のダイナミクスに関する情報の抽出を可能年、様々な、1分子分析、診断法の開発へとつながる基盤技術として期待される。2014年にノーベル化学賞が贈られた、超解像顕微鏡の開発においては、1つ1つ蛍光分子から点を描き、点描画のように高解像度の画像が得られる。本研究では、蛍光分子から点を描くだけでなく、各蛍光分子から発せられる情報をもマッピング可能な技術へと発展すると期待される。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (18 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] imec(ベルギー)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ETH Zurich/ University of Zurich(スイス)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Single-Molecule Study of Redox Reaction Kinetics by Observing Fluorescence Blinking2021

    • Author(s)
      Kawai Kiyohiko、Fujitsuka Mamoru、Maruyama Atsushi
    • Journal Title

      Accounts of Chemical Research

      Volume: 54 Issue: 4 Pages: 1001-1010

    • DOI

      10.1021/acs.accounts.0c00754

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of Single‐Stranded RNA Looping Probed and Photoregulated by Sulfonated Pyrene2020

    • Author(s)
      J. Xu, S. Tojo, M. Fujitsuka and K. Kawai
    • Journal Title

      ChemistrySelect

      Volume: 5 Issue: 26 Pages: 8002-8008

    • DOI

      10.1002/slct.202002231

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Kinetics of Photoinduced Reactions at the Single‐Molecule Level: The KACB Method2020

    • Author(s)
      Kawai Kiyohiko、Maruyama Atsushi
    • Journal Title

      Chemistry - A European Journal

      Volume: 26 Issue: 35 Pages: 7740-7746

    • DOI

      10.1002/chem.202000439

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sulfonated Pyrene as a Photoregulator for Single-Stranded DNA Looping2020

    • Author(s)
      Jie Xu, Shunichi Miyamoto, Sachiko Tojo and Kiyohiko Kawai
    • Journal Title

      Chemistry - A European Journal

      Volume: 26 Issue: 22 Pages: 5075-5084

    • DOI

      10.1002/chem.202000184

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single-Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking2017

    • Author(s)
      Kawai Kiyohiko、Miyata Takafumi、Shimada Naohiko、Ito Syoji、Miyasaka Hiroshi、Maruyama Atsushi
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 56 Issue: 48 Pages: 15329-15333

    • DOI

      10.1002/anie.201708705

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Sulfonated Pyrene as Both a Probe and a Photoregulator for Single-Stranded Oligonucleotide Looping2020

    • Author(s)
      Jie, Xu; Shunichi, Miyamoto; Sachiko, Tojo; Mamoru Fujitsuka; Kiyohiko Kawai
    • Organizer
      光化学討論会2020
    • Related Report
      2019 Annual Research Report
  • [Presentation] 核酸1分子を見つける、調べる2019

    • Author(s)
      川井 清彦
    • Organizer
      第22回生命化学研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 蛍光blinking制御によるRiboswitch構造変化の1分子観測2019

    • Author(s)
      川井 清彦
    • Organizer
      日本分析化学会第68回年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Single-Molecule Level Monitoring of Nucleic Acids Conformational Changes by Controlling the Fluorescence Blinking2019

    • Author(s)
      Kiyohiko Kawai
    • Organizer
      π-System Figuration European-Japanese Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-Molecule Level Analysis of Nucleic Acids Structure by Controlling the Blinking2019

    • Author(s)
      Kiyohiko Kawai, Takafumi Miyata, Naohiko Shimada, Atsushi Maruyama
    • Organizer
      ISNAC 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] rKACB法による核酸構造の1分子分析2019

    • Author(s)
      川井 清彦、宮田 貴史、嶋田 直彦、丸山 厚
    • Organizer
      第13回バイオ関連化学シンポジウム2019
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光のblinking観測による核酸構造の1分子分析2018

    • Author(s)
      川井清彦
    • Organizer
      日本化学会 第98春季年会 (2018)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] DNA STRUCTURAL CHANGES MONITORED BY CONTROLLING THE FLUORESCENCE BLINKING2017

    • Author(s)
      川井 清彦、丸山 厚、真嶋 哲朗
    • Organizer
      The 15th Conference on Methods and Applications in Fluorescence
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Book] 核酸科学ハンドブック2020

    • Author(s)
      日本核酸化学会、杉本 直己
    • Total Pages
      576
    • Publisher
      講談社
    • ISBN
      9784065207864
    • Related Report
      2019 Annual Research Report
  • [Remarks] 研究紹介: 現象を調べ、わかったことを積極的に活用することを目指しています!

    • URL

      https://www.sanken.osaka-u.ac.jp/labs/mec/kawairesearch.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] Single-molecule level analysis

    • URL

      https://www.sanken.osaka-u.ac.jp/labs/mec/kawairesearch-e.html

    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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