| Budget Amount *help |
¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
Magic-angle spinning solid-state NMR methods has recently witnessed a maturation of methodologies utilizing paramagnetic dopants for accelerating signal averaging and long-range internuclear distance measurements. These original methods had however some own limitations coming from the paramagnetism that remains during the signal acquisition period, resulting in line broadening or signal loss. In this study, light-excitable transiently paramagnetic species were explored to make a functional dopants and protein-tag whose magnetism is switchable by making on/off the excitation light. This is shown here to resolve the conventional methodological limitations, which will become a basis for new and more efficient tools for structural study of proteins.
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