Temporal and Spatial Active-Control of Mass Diffusion for High Quality Crystallization of Protein

• Project/Area Number: 17H03181
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thermal engineering
• Research Institution: Tohoku University
• Principal Investigator: Komiya Atsuki 東北大学, 流体科学研究所, 教授 (60371142)
• Co-Investigator(Kenkyū-buntansha): 岡島 淳之介 東北大学, 流体科学研究所, 助教 (70610161)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: タンパク質 / 物質拡散 / 結晶成長 / 位相シフト干渉計 / 機能性膜

Outline of Final Research Achievements

This research focused on the proposal of high-quality crystallization method for proteins by active control of protein mass transfer in liquid phase. Applying a special membrane which has micro-structured pores, a hindered diffusion field in the vicinity of membrane was experimentally visualized and the possibility of its active control was evaluated. Numerical simulation of transient diffusion field was also performed, and quantitative evaluation of mass diffusion process was done. Through the results from experiment and numerical simulations, the key factor for actively controlling protein mass transfer was evaluated.

Academic Significance and Societal Importance of the Research Achievements

本研究では，研究目的にも示したように，タンパク質高品位結晶化の実現を目指している．得られた研究成果から，自然対流が抑制された条件下で容易にタンパク質の高品質結晶を創り出すことが期待でき，タンパク質の分子構造情報を高精度に取得することが可能となる．これによりタンパク質の物性や機能の理解が進み，学術的には創薬や食品学の分野の発展に資する情報が提供でき，また，高品質の薬品等が効率よく生産できることから，社会的意義も大きいものとなる．

Research Products

• [Int'l Joint Research] INSA Lyon(フランス)
• [Journal Article] Law of the wall for a temporally evolving vertical natural convection boundary layer (2020)
  • Author(s): Junhao ke、Nicholas Williamson、Steve W. Armfield、Stuart E. Norris、Atsuki Komiya
  • Journal Title: Journal of Fluid Mechanics Volume: 902
  • DOI: 10.1017/jfm.2020.621
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Optical Method for Simultaneous High-Resolution Measurement of Heat and Fluid Flow: The Case of Rayleigh-B?nard Convection (2020)
  • Author(s): Juan F. Torres、Yongling Zhao、Shuqi Xu、Zhengyu Li、Atsuki Komiya
  • Journal Title: Physical Review Applied Volume: 14 Issue: 5 Pages: 054038-054038
  • DOI: 10.1103/physrevapplied.14.054038
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Three-step phase-shifting imaging ellipsometry to measure nanofilm thickness profiles (2019)
  • Author(s): Eita Shoji, Atsuki Komiya, Junnosuke Okajima, Masaki Kubo, Takao Tsukada
  • Journal Title: Optics and Lasers in Engineering Volume: 112 Pages: 145-150
  • DOI: 10.1016/j.optlaseng.2018.09.005
  • Peer Reviewed
• [Presentation] Experimental Evaluation of Objective Function for Perdition of IR Camera's Sensitivity (2020)
  • Author(s): Takuma Kogawa, Junnosuke Okajima, Atsuki Komiya and Shigenao Maruyama
  • Organizer: 20th International Symposium on Advanced Fluid Information (AFI-2020)
  • Int'l Joint Research
• [Presentation] Integral Modelling of an Unsteady Natural Convection Boundary Layer (2020)
  • Author(s): Junhao Ke, Nicholas Williamson, Steven W. Armfield and Atsuki Komiya
  • Organizer: 20th International Symposium on Advanced Fluid Information (AFI-2020)
  • Int'l Joint Research
• [Presentation] Quantitative Visualization of Flow Bifurcation in Laminar Rayleigh-Bénard Convection with Combined PSI and PIV (2020)
  • Author(s): Shuqi Xu, Yongling Zhao, Atsuki Komiya and Juan Felipe Torres Alvarez
  • Organizer: 20th International Symposium on Advanced Fluid Information (AFI-2020)
  • Int'l Joint Research
• [Presentation] Effect of Micropores Patterning in Separated Plate on Protein Hindered Diffusion Phenomena (2020)
  • Author(s): Atsuki Komiya, Ryo Watanabe, Yuki Kanda, Juan Felipe Torres Alvarez and Sebastien Livi
  • Organizer: 20th International Symposium on Advanced Fluid Information (AFI-2020)
  • Int'l Joint Research
• [Presentation] マイクロ細孔を利用したタンパク質の物質拡散制御の可能性評価 (2020)
  • Author(s): 渡邉崚, Juan Felipe Torres Alvarez, 神田雄貴, 小宮敦樹
  • Organizer: 日本機械学会熱工学コンファレンス2020
• [Presentation] Evaluation of the Relations between Hindered Diffusion Process of Protein and Membrane Structure (2019)
  • Author(s): Atsuki Komiya, Ryo Watanabe, Hani Alkitabi Aldaftari, Shuichi Moriya and Sebastien Livi
  • Organizer: The Second Pacific Rim Thermal Engineering Conference
  • Int'l Joint Research
• [Presentation] Measurement of protein mass flux through porous membrane (2019)
  • Author(s): Ryo Watanabe, Shuichi Moriya, Sebastien Livi and Atsuki Komiya
  • Organizer: ELyT Workshop 2019
  • Int'l Joint Research
• [Presentation] Quantitative Evaluation of the Influence of Membrane Structure on Protein Mass Diffusion (2019)
  • Author(s): Ryo Watanabe, Shuichi Moriya, Sebastien Livi, Hani Alkitabi Aldaftari and Atsuki Komiya
  • Organizer: 16th International Conference on Flow Dynamics
  • Int'l Joint Research
• [Presentation] Numerical Simulation of Concentration Field around a Growing Crystal Subjected to Gravity (2019)
  • Author(s): Hani Alkitabi Aldaftari, Ryo Watanabe and Atsuki Komiya
  • Organizer: 16th International Conference on Flow Dynamics
  • Int'l Joint Research
• [Presentation] Protein Mass Transfer Control by Using Hindered Diffusion in Membrane (2019)
  • Author(s): Atsuki Komiya, Ryo Watanabe and Sebastien Livi
  • Organizer: 19th International Symposium on Advanced Fluid Information
  • Int'l Joint Research
• [Presentation] Experimental Study on Active Control of Protein Mass Flux by Functional Membrane (2018)
  • Author(s): Atsuki Komiya and Sebastien Livi
  • Organizer: ELyT Workshop 2018
  • Int'l Joint Research
• [Presentation] Experimental Study on Active Control of Protein Mass Flux by Functional Membrane (2018)
  • Author(s): Atsuki Komiya and Sébastien Livi
  • Organizer: ELyT Workshop 2018
  • Int'l Joint Research
• [Presentation] Experimental Study on Active Control of Protein Transport Phenomena by Membrane (2017)
  • Author(s): Atsuki Komiya and Sébastien Livi
  • Organizer: The 28th International Symposium on Transport Phenomena (ISTP-28)
  • Int'l Joint Research