| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
In recent years, the pandemic of the new coronavirus has become a major threat, and the development of rapid and simple DNA diagnostic technology has become an issue. In this research, we aimed to improve the sensitivity of DNA diagnostic technology based on a new principle using traveling wave electric field. First, we developed a system for quantifying the velocity of individual microbeads by observing the behavior of many DNA-labeled microbeads under a traveling wave electric field. As a result, it was clarified that the movement speed of microbeads increased with the increase of the amount of the labeling DNA. Regarding the DNA detection sensitivity by the developed method, the amount of labeling DNA was 100 DNA molecules/ bead. This is about 1000 times more sensitive than the DNA diagnostic method developed by the group of investigators in the past.
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