| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
In this study, we developed a new method for the preparation of membrane proteins using magnetic bacteria as a host, which can be directly used for drug discovery without the need for complicated processes such as purification of membrane proteins or preparation of reconstituted membranes. The lipid membrane covering the magnetosomes (magnetic particles) biosynthesized by magnetic bacteria was used as a platform for expression of membrane proteins, and the functional expression of human-derived membrane proteins was realized by modifying the composition of the membrane using a genetic engineering method. The human-derived membrane protein-magnetic particle complexes created by this method can be applied to innovative methods to realize high-throughput drug discovery research.
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