| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
(1) Based on stereospecific targeting (SST) technique, we succeeded in producing conformation-specific monoclonal antibodies against membranous proteins with high efficiency, regardless of that it has been known to be quite difficult for generating them. (2) EGFR-recognizing and Swine Influenza Virus-binding rabbit monoclonal antibodies were obtained by using the Ecobody technology, directly from single B cells. In addition, a sand witch detection system for the virus was also constructed.(3) The formation of pairs of the different types of cells and the electrofusion of pairs were achieved by using the novel device with microband electrodes based on dielectrophoresis. (4) We developed the method for preparation of membrane protein-displaying artificial lipid membranes supported on spherical silica microbeads using direct membrane fusion with recombinant baculovirus virions expressing GPCRs like adrenergic receptors.
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