| Project/Area Number |
17H03471
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Nakamura Chikashi 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (40357661)
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| Co-Investigator(Kenkyū-buntansha) |
山岸 彩奈 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (00778293)
長崎 晃 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30392640)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | バイオセンサー / がん細胞 / 中間径フィラメント / 抗体 / 細胞骨格 / 細胞・組織 / 癌 / ナノバイオ / 走査型プローブ顕微鏡 / 走査プローブ顕微鏡 |
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| Outline of Final Research Achievements |
In this study, the structure of intermediate filament (IF) nestin as a skeleton in highly metastatic mouse breast cancer cells and the mechanical functions were analyzed. In this strain, nestin forms copolymerized fibers with vimentin, and nestin deficiency caused increment of cell stiffness. The results of the mutant rescue showed that the C-terminal tail deleted nestin cannot restore the stiffness since the steric hindrance with the tail domain might prevent vimentin-actin binding. The results of mechanical tests using AFM showed that nestin has the ability to increase the mobility of IF. These results suggested that the network structure of the cytoskeleton altered the cellular stiffness, resulting the malignancy of cancer cells.
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| Academic Significance and Societal Importance of the Research Achievements |
開発する技術は、申請者が独自に開発してきた免疫力学測定法をさらに深化させたものであり、生きた細胞の骨格蛋白質の機械的特性を評価できるという点で極めて独創性が高く、細胞生物学にブレークスルーをもたらす基盤技術となると考えている。また本技術は、癌細胞の転移メカニズムの解明をはじめ、細胞病理学における重要な知見を与えるものと期待できる。
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