Super resolution live imaging of synaptic vesicle at neuronal presynaptic terminal

• Project/Area Number: 17H03548
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Midorikawa Mitsuharu 東京女子医科大学, 医学部, 講師 (60632643)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2020: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: シナプス / 開口放出 / ライブイメージング / 電気生理 / 電気生理学 / イメージング / 神経科学 / 生理学 / 脳・神経

Outline of Final Research Achievements

By applying super resolution live imaging to presynaptic terminals at central nervous system, we found that the kinetics of synaptic vesicles were diverse among synapses with distinct functions. We also found some detailed presynaptic mechanisms underlies the plastic changes of the synaptic functions, which is assumed to be crucial for remodeling of the neuronal circuit following learning or development.

Academic Significance and Societal Importance of the Research Achievements

シナプス小胞からの神経伝達物質の放出機構は、中枢神経回路において神経細胞同士の情報伝達を担う重要な細胞機能であり、その詳細を明らかにすることは脳機能を理解する上で避けては通れない命題である。本研究による成果は様々な細胞からの多様な神経伝達物質放出様式を可能にしている基盤現象を示したものであり、将来的にはこれらの異常を原因とする様々な精神疾患の原因究明や治療戦略の創出の萌芽となることが期待される。

Research Products

• [Journal Article] Distinct functional developments of surviving and eliminated presynaptic terminals (2021)
  • Author(s): Midorikawa M, Miyata M.
  • Journal Title: Proc Natl Acad Sci U S A . Volume: 118 Issue: 11
  • DOI: 10.1073/pnas.2022423118
  • NAID: 120007147680
  • Peer Reviewed / Open Access
• [Journal Article] 中枢神経シナプス前終末における小胞動態の 超解像ライブイメージング (2019)
  • Author(s): 緑川光春
  • Journal Title: 月刊「細胞 The CELL」 Volume: 12月号 Pages: 37-40
  • Peer Reviewed
• [Journal Article] Real-time imaging of synaptic vesicle exocytosis by total internal reflection fluorescence (TIRF) microscopy. (2018)
  • Author(s): Midorikawa M.
  • Journal Title: Neurosci Res. Volume: S0168-0102(17) Pages: 30755-1
  • DOI: 10.1016/j.neures.2018.01.008
  • Peer Reviewed
• [Journal Article] Kinetics of Releasable Synaptic Vesicles and Their Plastic Changes at Hippocampal Mossy Fiber Synapses. (2017)
  • Author(s): Midorikawa M, Sakaba T.
  • Journal Title: Neuron Volume: 96 Issue: 5 Pages: 1033-1040
  • DOI: 10.1016/j.neuron.2017.10.016
  • Peer Reviewed
• [Presentation] Sensory input dependent and independent development of presynaptic transmitter release mechanisms at lemniscal fiber terminals in the somatosensory thalamus (2021)
  • Author(s): 緑川光春、宮田麻理子
  • Organizer: 第126回日本解剖学会総会・全国学術集会 / 第98回日本生理学会大会 合同大会
• [Presentation] Direct measurements of transmitter release kinetics at lemniscal fiber terminals in the somatosensory thalamus. (2020)
  • Author(s): 緑川光春、宮田麻理子
  • Organizer: 第97回日本生理学会大会
• [Presentation] Direct measurements of transmitter release from lemniscal fiber terminals in the somatosensory thalamus. (2019)
  • Author(s): 緑川光春、宮田麻理子
  • Organizer: 第42回日本神経科学大会
• [Presentation] Presynaptic properties at lemniscal fiber terminals in the somatosensory thalamus (2019)
  • Author(s): 緑川光春、宮田麻理子
  • Organizer: 第9回アジア・オセアニア生理学会連合 (FAOPS) 2019年大会
  • Int'l Joint Research / Invited
• [Presentation] Investigating presynaptic properties of afferent fibers in the somatosensory thalamus. (2018)
  • Author(s): 緑川光春、宮田麻理子
  • Organizer: Core-to-Core program “Nanobiology of neural plasticity based on optical nanoscopy”
• [Presentation] Measuring dynamics of releasable synaptic vesicles at hippocampal mossy fiber boutons. (2018)
  • Author(s): 緑川光春、坂場武史
  • Organizer: COLD SPRING HARBOR ASIA CONFERENCES
  • Int'l Joint Research
• [Presentation] 中枢神経シナプス前終末における単一シナプス小胞動態の可視化 (2018)
  • Author(s): 緑川光春、坂場武史
  • Organizer: 第95回日本生理学会大会
• [Remarks] 東京女子医科大学 医学部 生理学 （神経生理学分野）ホームページ
  • URL: http://www.twmu.ac.jp/neurophysiology/
• [Remarks] 東京女子医科大学ホームページ
  • URL: http://www.twmu.ac.jp/univ/news/?kbn=1&ym=202103