Elucidation of mechanisms underlying colorectal tumorigenesis associated with deregulation of new Wnt targets
Project/Area Number |
17H03575
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
山口 貴世志 東京大学, 医科学研究所, 特任講師 (50466843)
池上 恒雄 東京大学, 医科学研究所, 准教授 (80396712)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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Keywords | がん / シグナル伝達 / Wnt |
Outline of Final Research Achievements |
In this study, we identified new target genes regulated by the canonical Wnt signaling. Among the genes, FRMD5 was positively regulated, and IFIT2 was negatively regulated by the signaling. Elevated expression of FRMD5 is associated with invasion of colorectal cancer (CRC) cells, and high FRMD5 expression in CRC tissues is linked to poor prognosis. On the other hand, reduced IFIT2 expression is associated with cell proliferation and resistance to apoptosis. Furthermore, we identified IRF1 as a transcriptional regulator of IFIT2. Importantly, we revealed that activation of the Wnt signaling downregulates IRF1 through its destabilization, and that a deubiquitinating enzyme USP1 plays an important role in the stabilization of IRF1. We also found that UAF1, a USP-interacting protein, is reduced by the activated Wnt signaling. These data suggest that activation of the signaling reduces the stability of IRF1 through its ubiquitination by suppression of USP1-UAF1 activity.
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果は大腸がん発生・進展メカニズムの理解に役立つのみならず、新たなバイオマーカーや治療薬開発などに応用できるかもしれない。学術面では、Wnt活性化により特定のタンパク質分解が誘導されるという発見は、これまで知られていない新たなWntシグナル制御機構で、他にも制御される分子が存在する可能性が考えられる。社会的意義ではFRMD5の発現と臨床情報の詳細な検討により、FRMD5が新規予後マーカーとして利用できる可能性が示された。またWnt阻害によって発現が増加するIFIT2やIRF1は、Wntを阻害する化合物、抗体等の探索プローブとしての応用が可能であり、今後の治療薬開発に役立つかもしれない。
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Development of an MSI-positive colon tumor with aberrant DNA methylation in a PPAP patient2019
Author(s)
Yamaguchi K, Shimizu E, Yamaguchi R, Imoto S, Komura M, Hatakeyama S, Noguchi R, Takane K, Ikenoue T, Gohda Y, Yano H, Miyano S, Furukawa Y
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Journal Title
J Hum Genet.
Volume: 64(8)
Issue: 8
Pages: 729-740
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Importance of Gastric Cancer for the Diagnosis and Surveillance of Japanese Lynch Syndrome Patients2019
Author(s)
Ikenoue T, Arai M, Ishioka C, Iwama T, Kaneko S, Matsubara N, Moriya Y, Nomizu T, Sugano K, Tamura K, Tomita N, Yoshida T, Sugihara K, Naruse H, Yamaguchi K, Nojima M, Nakamura Y, Furukawa Y; Japanese society for cancer of the colon and rectum (JSCCR)
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Journal Title
J Hum Genet.
Volume: 64
Issue: 12
Pages: 1187-1194
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Deep sequencing across germline GWAS signals relating to breast cancer events in women receiving aromatase inhibitors for adjuvant therapy of early breast cancer.2019
Author(s)
Ingle JN, Kalari KR, Momozawa Y, Kubo M, Furukawa Y, Shepherd LE, Ellis MJ, Goss PE, Barman P, Carlson EE, Sinnwell JP, Tang X, Goetz MP, Chen BE, Cairns J, Weinshilboum RM, Wang L
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Journal Title
Pharmacogenetics and Genomics
Volume: 29(8)
Issue: 8
Pages: 183-191
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Development of an MSI-positive colon tumor with aberrant DNA methylation in a PPAP patient2019
Author(s)
Yamaguch K, Shimizu E, Yamaguchi R, Imoto S, Komura M, Hatakeyama S, Noguchi R, Takane K, Ikenoue T, Gohda Y, Yano H, Miyano S, Furukawa Y.
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Journal Title
Journal of Human Genetics
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Efficacy of liquid-based genetic diagnosis of endometrial cancer.2018
Author(s)
Matsuura M, Yamaguchi K, Tamate M, Satohisa S, Teramoto M, Iwasaki M, Sugita S, Hasegawa T, Koubo R, Takane K, Ikenoue T, Furukawa Y, Saito T.
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Journal Title
Cancer Science
Volume: 109
Issue: 12
Pages: 4025-4032
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Fbxo22-mediated KDM4B degradation determines selective estrogen receptor modulator activity in breast cancer.2018
Author(s)
Johmura Y, Maeda I, Suzuki N, Wu W, Goda A,Morita M, Yamaguchi K, Yamamoto M, Nagasawa S, Kojima Y, Tsugawa K, Inoue N, Miyoshi Y, Osako T Akiyama F, Maruyama R, Inoue JI, Fukukawa Y, Ohta T, Nkanishi M.
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Journal Title
J Clin Invest.
Volume: 128
Issue: 12
Pages: 5603-5619
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genetic variation determines VEGF-A plasma levels in cancer patients.2018
Author(s)
Innocenti F, Jiang C, Sibley AB, Etheridge AS, Hatch AJ, Denning S, Niedzwiecki D, Shterev ID, Lin J, Furukawa Y, Kubo M, Kindler HL, Auman JT, Venook AP, Hurwitz HI, McLeod HL, Ratain MJ, Gordan R, Nixon AB, Owzar K.
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 16332-16332
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Germline genome-wide association studies in women receiving neoadjuvant chemotherapy with or without bevacizumab.2018
Author(s)
Ingle JN, Kalari KR, Wickerham DL, von Minckwitz G, Fasching PA, Furukawa Y, Mushiroda T, Goetz MP, Barman P, Carlson EE, Rastogi P, Costantino JP, Cairns J, Paik S, Bear HD, Kubo M, Wang L, Wolmark N, Weinshilboum RM.
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Journal Title
Pharmacogenet Genomics
Volume: 28
Issue: 6
Pages: 147-152
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Whole-exome sequencing of high-grade fetal lung adenocarcinomas2019
Author(s)
Masaki Suzuki, Rika Kasajima, Tomoyuki Yokose, Rui Yamaguchi, Seiya Imoto, Yoichi Furukawa, Satoru Miyano3 Emi Yoshioka, Kota Washimi, Yoichiro Okubo, Kae Kawachi, Yohei Miyagi
Organizer
The 16th Korean-Japanese Conjoint Slide Conference of International Academy of Pathology
Related Report
Int'l Joint Research
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