| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
Constitutive activation of Wnt signaling is observed in most colorectal cancers (CRCs) and has been believed to play a pivotal role in maintaining cancer stem cells. Through the screening with a siRNA library targeting 70 molecules previously identified as components of the TCF4/β-catenin transcriptional complex, we have explored their regulatory effects on the Wnt signaling. We looked at the expression of Wnt target genes such as c-Myc and Axin, cell proliferation, and Wnt signaling activities in colorectal cancer spheroids by WASA (Wnt signaling Activity in Spheroid Assay) method we developed. As a result, we identified several molecules as candidates for therapeutic targets for CRCs. Of interest, most of the candidate molecules were involved in DNA damage and transcription-coupled DNA repair.
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