| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Large numbers of non-protein-coding RNAs are transcribed from the genomes of higher organisms including human and mouse. In this study, we aimed to systematically select functional lncRNA candidate genes and to establish an efficient method for functional analyses. We found that functional lncRNAs that form tight complexes with proteins can be predicted based on the amount of RNAs recovered from the aqueous phase after UV irradiation. We also established a method to efficiently generate mutant mice lacking lncRNA function using the iGONAD method, a recently developed method that enables a low-cost, high-efficient genome editing.
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