| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
The goal of this study is to establish a method to identify the endogenous kinase substrates on a large scale, and to apply to intracellular signal pathway analysis. To accomplish this goal, we have developed three methods as follows: (1) a method to measure changes in phosphorylation upon kinase perturbation, (2) a method to calculate the motif score based on the extracted phosphorylation motif of each kinase from in vitro kinase assay, and (3) a method to analyze the kinase-substrate transient interactions. These three methods were combined and applied to identifying endogenous kinase substrates successfully. In addition, we developed a method for estimating the activated kinase from the phosphoproteome information based on kinase-substrate relationships.
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