Skeletal muscle differentiation regulated by chromatin composition
Project/Area Number |
17H03608
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | Kyushu University |
Principal Investigator |
Ohkawa Yasuyuki 九州大学, 生体防御医学研究所, 教授 (80448430)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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Keywords | クロマチン / ヒストンバリアント / 骨格筋分化 / 転写調節 / エピゲノム解析 / 発現制御 / 遺伝子 / 発生・分化 / エピゲノム / クロマチン構造 / 細胞分化 |
Outline of Final Research Achievements |
In cell differentiation, a group of differentiation-related genes are selectively expressed by various epigenome regulations including transcription factors and histone modifications. In recent years, it has been cleared that histone variants form a chromatin structure at a specific composition ratio and are widely involved in the selective gene expression on the genome to the regulation of transcriptional amount. However, there are still many unclear points about the formation mechanism and function. Therefore, in the present study, novel identified skeletal muscle-specific histone variants from the selection of differentiation genes in the process of reaching various precursor cells and mature muscle tissues in the process from skeletal muscle stem cells to mature muscle tissues. We have elucidated the chromatin composition on the genome and its function when controlling the transcription level, some of which have been published and others have been submitted.
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、骨格筋幹細胞から筋肉を形成する遺伝子発現プログラムの解明を進めた。細胞内の遺伝子発現は、クロマチン構造の変換により制御されている。その構造を担っているのが、ヒストンである。本研究ではヒストンの亜種の解析、特に骨格筋幹細胞に発現するものに焦点を絞って解析を行い、骨格筋分化を促進、あるいは阻害する機能をもつ新規ヒストンを同定した。今後これらヒストンを起点とした筋再生機構の解明や応用が期待できる。
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Report
(4 results)
Research Products
(89 results)
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[Journal Article] Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle.2019
Author(s)
Fukuda S, Kaneshige A, Kaji T, Noguchi YT, Takemoto Y, Zhang L, Tsujikawa K, Kokubo H, Uezumi A, Maehara K, Harada A, Ohkawa Y, Fukada SI.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Regulation of ectopic heterochromatin-mediated epigenetic diversification by the JmjC family protein Epe1.2019
Author(s)
Sorida M, Hirauchi T, Ishizaki H, Kaito W, Shimada A, Mori C, Chikashige Y, Hiraoka Y, Suzuki Y, Ohkawa Y, Kato H, Takahata S, Murakami Y
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Journal Title
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] CLEC3A, MMP7, and LCN2 as Novel Markers for Predicting Recurrence in Resected G1 and G2 Pancreatic Neuroendocrine Tumors2019
Author(s)
Miki M, Oono T, Fujimori N, Takaoka T, Kawabe K, Miyasaka Y, Ohtsuka T, Saito D, Nakamura M, Ohkawa Y, Oda N, Suyama M, Ito T, Ogawa Y.
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Journal Title
Cancer Medicine
Volume: in press
Issue: 8
Pages: 3748-3760
DOI
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Peer Reviewed / Open Access
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[Journal Article] miR-124 dosage regulates prefrontal cortex function by dopaminergic modulation.2019
Author(s)
Kozuka T, Omori Y, Watanabe S, Tarusawa E, Yamamoto H, Chaya T, Furuhashi M, Morita M, Sato T, Hirose S, Ohkawa Y, Yoshimura Y, Hikida T, Furukawa T.
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Journal Title
Sci Rep.
Volume: Mar 5;9(1)
Issue: 1
Pages: 3445-3445
DOI
NAID
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Peer Reviewed / Open Access
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[Journal Article] Ad4BP/SF-1 regulates cholesterol synthesis to boost the production of steroids2018
Author(s)
Takashi Baba, Hiroyuki Otake, Miki Inoue, Tetsuya Sato, Yasuhiro Ishihara, Ju-Yeon Moon, Megumi Tsuchiya, Kanako Miyabayashi, Hidesato Ogawa, Yuichi Shima, Lixiang Wang, Ryuichiro Sato, Takeshi Yamazaki, Mikita Suyama, Masatoshi Nomura, Man Ho Choi, Yasuyuki Ohkawa & Ken-ichirou Morohashi
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Journal Title
Communications Biology
Volume: 1
Issue: 1
Pages: 1-9
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Histone methyltransferase G9a is a key regulator of the starvation-induced behaviors in Drosophila melanogaster.2017
Author(s)
Shimaji, K., Tanaka, R., Maeda, T., Ozaki, M., Yoshida, H., Ohkawa, Y., Sato, T., Suyama, M. and Yamaguchi, M.
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 14763-14763
DOI
NAID
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Peer Reviewed / Open Access
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[Journal Article] Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.82017
Author(s)
Taguchi H, Xie Y, Horikoshi N, Maehara K, Harada A, Nogami J, Sato K, Arimura Y, Osakabe A, Kujirai T, Iwasaki T, Semba Y, Tachibana T, Kimura H, Ohkawa Y, Kurumizaka H.
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Journal Title
Biochemistry
Volume: 56
Issue: 16
Pages: 2184-2196
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Thymine DNA glycosylase modulates the DNA damage response and gene expression by base excision repair-dependent and -independent mechanisms2017
Author(s)
Tomohumi Nakamura, Kouichi Murakami, Haruto Tada, Yoshihiko Uehara, Jumpei Nogami, Kazumitsu Maehara, Yasuyuki Ohkawa, Hisato Saitoh, Hideo Nishitani, Tetsuya Ono, Ryotaro Nishi, Masayuki Yokoi, Wataru Sakai, and Kaoru Sugasawa
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Journal Title
Genes to Cells
Volume: 22
Issue: 4
Pages: 392-405
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Crystal structure of the overlapping dinucleosome composed of hexasome and octasome2017
Author(s)
Daiki Kato, Akihisa Osakabe, Yasuhiro Arimura, Yuka Mizukami, Naoki Horikoshi, Kazumi Saikusa, Satoko Akashi, Yoshifumi Nishimura, Sam-Yong Park, Jumpei Nogami, Kazumitsu Maehara, Yasuyuki Ohkawa, Atsushi Matsumoto, Hidetoshi Kono, Rintaro Inoue, Masaaki Sugiyama, Hitoshi Kurumizaka
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Journal Title
Science
Volume: 356
Issue: 6334
Pages: 205-208
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Book] 実験医学2019
Author(s)
原田 哲仁,大川 恭行
Total Pages
6
Publisher
羊土社
Related Report
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[Patent(Industrial Property Rights)] DNA結合タンパク質の結合領域の近傍に所望のDNA断片を挿入する方法2017
Inventor(s)
大川恭行,原田哲仁,胡桃坂仁志,木村宏,半田哲也,他2名
Industrial Property Rights Holder
大川恭行,原田哲仁,胡桃坂仁志,木村宏,半田哲也,他2名
Industrial Property Rights Type
特許
Filing Date
2017
Related Report
Overseas