| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
Although sexual reproduction is prevailing among eukaryotes, the nematode Diploscapter coronatus, a close relative of the model organism, C. elegans, reproduces parthenogenetically. Neither males nor sperm have been observed and some steps of meiosis are apparently skipped in this species. Our draft genome sequencing showed that there was about 6% heterozygosity between the diploid genome, which consists of two chromosomes (2n=2). Many genes involved in sex determination and meiosis are missing or very divergent.
In this study we carried out the phasing of the genome taking advantages of long read sequencing technology including PacBio and Irys to obtain clues to understand the evolution of the genome. The relationship of gene arrangements with the C. elegans genome suggests complex process of the genome evolution including chromosome fusion. Genome comparison with other close relatives will provide more clues to understand the basis for the occurrence of parthenogenesis.
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