| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
Thus far, presence of a significant number of mutations has been reported in iPS cell genomes. In this study, we aimed to understand the mechanism which causes these numerous mutations and develop a method to generate iPS cells with few mutations. In this study, we demonstrated that the initial stage of genome reprogramming is radio resistance. Further analyses revealed a overexpression of CyclinD1 and a deficiency of cell cycle check point function occur in a transient manner during the initial stage of genome reprogramming. Importantly, it was showed that the point mutations in iPS cell genomes accumulate just in the initial stage. In addition, we here also discovered that iPS cells established from human cord blood-derived erythroblasts had a mutation frequency of 1/5 to 1/10 of the frequency observed in previous iPS cells.
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