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Systematic analysis of proteins that cause overload of transport resources due to overexpression

Research Project

Project/Area Number 17H03618
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field System genome science
Research InstitutionOkayama University

Principal Investigator

Moriya Hisao  岡山大学, 環境生命科学研究科, 准教授 (60500808)

Co-Investigator(Kenkyū-buntansha) 牧野 能士  東北大学, 生命科学研究科, 教授 (20443442)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2017: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords細胞 / 過剰発現 / 細胞内輸送 / 酵母 / 増殖阻害 / ゲノム / タンパク質
Outline of Final Research Achievements

Overexpression of certain proteins inhibits cell proliferation. However, the mechanism of this is largely unknown. Our previous studies have shown that the depletion of essential factors due to an overload of proteins transported in the cell--transportation overload--may explain most of the growth inhibition caused by overexpression. In this study, we aimed to elucidate the mechanism of growth inhibition by transport overload through the identification of proteins that cause transport overload and restriction factors that are targets of overload. As a result, we identified several candidate limiting factors for nuclear transport in particular.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、特定のオルガネラや細胞内にタンパク質を大量生産したい場合の、積み荷タンパク質や宿主細胞の改変に利用できる可能性がある。また、癌細胞で過剰発現しているタンパク質の性質から輸送の制限因子への過負荷を予測できれば、その制限因子を標的とすることで癌細胞のみを選択的に死滅させるような、新しい治療方法を提案できる可能性がある。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (20 results)

All 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (10 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Development of an experimental method of systematically estimating protein expression limits in HEK293 cells2020

    • Author(s)
      Mori Yoshihiro、Yoshida Yuki、Satoh Ayano、Moriya Hisao
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1

    • DOI

      10.1038/s41598-020-61646-3

    • NAID

      120006883502

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The expression level and cytotoxicity of green fluorescent protein are modulated by an additional N-terminal sequence2020

    • Author(s)
      Moriya Hisao、Graduate School of Environmental and Life Sciences, Research Core for Interdisciplinary Sciences, Okayama University
    • Journal Title

      AIMS Biophysics

      Volume: 7 Issue: 2 Pages: 121-132

    • DOI

      10.3934/biophy.2020010

    • NAID

      120006860108

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis122019

    • Author(s)
      Abe-Kanoh Naomi、Kunisue Narumi、Myojin Takumi、Chino Ayako、Munemasa Shintaro、Murata Yoshiyuki、Satoh Ayano、Moriya Hisao、Nakamura Yoshimasa
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 8866-8866

    • DOI

      10.1038/s41598-019-45248-2

    • NAID

      120006648600

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimating the Protein Burden Limit of Yeast Cells by Measuring Expression Limits of Glycolytic Proteins.2018

    • Author(s)
      Eguchi, Y., Makanae, K., Hasunuma, T., Ishibashi, Y., Kito, K., Moriya, H.
    • Journal Title

      Elife

      Volume: 7

    • DOI

      10.7554/elife.34595

    • NAID

      120006501235

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic Analysis of Signal Generation by the Rgt2 Glucose Sensor of Saccharomyces cerevisiae2018

    • Author(s)
      Scharff-Poulsen Peter、Moriya Hisao、Johnston Mark
    • Journal Title

      G3

      Volume: 8 Issue: 8 Pages: 2685-2696

    • DOI

      10.1534/g3.118.200338

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 過剰発現が適応的に働く遺伝子の体系的同定2019

    • Author(s)
      守屋央朗
    • Organizer
      日本進化学会第21回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Genetic profiling of protein burden and nuclear export overload2019

    • Author(s)
      金高令子、Charlie Boone、守屋央朗
    • Organizer
      ICYGMB 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Systematic Identification of Genes whose Overexpression Positively Affect Fitness in Various Environments2019

    • Author(s)
      佐伯望、守屋央朗
    • Organizer
      ICYGMB 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 過剰発現実験により探るプロテオームの拘束条件2019

    • Author(s)
      守屋央朗
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Systematic Identification of Genes Whose Overexpression Works Adaptively Using the ADOPT System2019

    • Author(s)
      佐伯望、守屋央朗
    • Organizer
      The 20th International Conference on Systems Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Defining harmful yeast oroteins by mesuring overexpression limits2018

    • Author(s)
      江口優一、守屋央朗
    • Organizer
      2018 Yeast Genetics Meeting
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] タンパク質の限界発現量から探る細胞の処理能力2018

    • Author(s)
      守屋央朗、加藤有香
    • Organizer
      酵母遺伝学フォーラム第51回研究報告会
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Presentation] 多コピー化が増殖に有利に働く酵母遺伝子のハイスループットスクリーニング2018

    • Author(s)
      佐伯望、江口優一、堀内智司、Herbst Konrad、Kirrmaier Daniel、Knop Michael 、守屋央朗
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Presentation] タンパク質の発現限界を測る遺伝学的手法TOW-Fu2018

    • Author(s)
      江口優一、守屋央朗
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Presentation] 過剰発現の限界から探る細胞の処理能力2018

    • Author(s)
      守屋央朗、江口優一、蒔苗浩司、加藤有香
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] 酵母とシステムバイオロジー(ブログ)

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HM_blog/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 岡山大学異分野融合先端研究コア・システム細胞学研究室

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HMlab/

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report 2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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