| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
|
|---|
| Outline of Final Research Achievements |
In order to elucidate the overall molecular mechanism of tardigrade anhydrobiosis, we first identified through multi-omics analysis that 1,422 genes are significantly induced in H. exemplaris which requires de novo gene expression for successful anhydrobiosis, and 928 of these to be conserved components, which we defined as the core gene set of anhydrobiosis. Time-series phosphoproteomics revealed that the induction of this drastic differential gene expression is regulated via AMPK signaling. Furthermore, we identified a novel gene, AMNP, involved in the repair from oxidative stress by the cross-tolerance assay of UV irradiation and anhyrobiosis. In addition, we found that liquid-liquid phase separation is involved in the mechanism of action of CAHS, and altogether elucidated the complex molecular mechanisms of tardigrade anhydrobiosis.
|
