| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
|
|---|
| Outline of Final Research Achievements |
The purpose of this study is to elucidate the interaction network between stress tolerance in bacteria and to develop a method to control the evolutionary dynamics. We performed laboratory evolution of E. coli cells under 95 stress environments and obtained stress-resistant strains. Then, we quantified gene expression profiles, genome mutations, and resistance profiles to various stress environments. Based on the data, we developed a method to control evolutionary dynamics by adding feedback regulations to selection environments. As a result, we successfully observed evolutionary dynamics toward target phenotypes, which cannot be obtained by the selection using single drugs.
|
