| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
|---|
| Outline of Final Research Achievements |
piRNAs are germ cell-specific functional small molecule RNAs that repress the expression of genes with complementary sequences. We identified an ATP-dependent RNA helicase, Armitage (Armi), as a piRNA biogenesis factor in Drosophila. By analyzing the RNA that binds to Armi in cells using iCLIP and CLIPPARE that we independently developed, we were able to capture the transition process of piRNA biogenesis, which has not been analyzed in detail. Biochemical analysis revealed a detailed molecular mechanism of the piRNA biogenesis pathway.
|
