Transcription-coupled pre-mRNA processing in living animal
| Project/Area Number |
17H03633
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 遺伝子発現制御 / 新生RNA / 線虫 / スプライシング / 転写後プロセシング / mRNA / 代謝標識 / RNA-seq解析 / RNA-seq / mRNA前駆体 / RNAプロセシング / 転写 / 組織特異性 / RNAseq / 遺伝子発現 |
|---|
| Outline of Final Research Achievements |
We developed new methods to label and purify nascent precursor messenger RNAs (pre-mRNAs) in a nematode worm C. elegans. With the methods, we revealed dynamics of pre-mRNA processing such as trans-splicing, cis-splicing and cleavage at a polyadenylation site for the first time in multicellular organisms. We also analyzed nascent pre-mRNAs in a splicing factor mutant and revealed effects of the factor on the dynamics of pre-mRNA processing.
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| Academic Significance and Societal Importance of the Research Achievements |
mRNA前駆体のプロセシング動態が多細胞生物の個体レベルで明らかになるのは初めてである。哺乳類ではイントロンが長大であることから、同様の方法を適用しても同様の結果を得ることは実質的に困難である。本研究成果は、哺乳類を含む他の多細胞動物における転写と共役したmRNAの転写後プロセシングによる遺伝子発現制御機構を考察する上で基礎となる重要な知見を与えるものである。
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization2018
Author(s)
Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura & Hidehito Kuroyanagi
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Journal Title
Scientific Reports
Volume: 印刷中
Issue: 1
Pages: 8970-8983
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization2018
Author(s)
Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura and Hidehito Kuroyanagi
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Journal Title
Scientific Reports
Volume: 印刷中
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Book] 遺伝子発現制御機構2017
Author(s)
田村隆明、浦聖惠（編）
Total Pages
264
Publisher
東京化学同人
ISBN
9784807909179
Related Report
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