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Transcription-coupled pre-mRNA processing in living animal

Research Project

Project/Area Number 17H03633
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUROYANAGI Hidehito  東京医科歯科大学, 難治疾患研究所, 准教授 (30323702)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords遺伝子発現制御 / 新生RNA / 線虫 / スプライシング / 転写後プロセシング / mRNA / 代謝標識 / RNA-seq解析 / RNA-seq / mRNA前駆体 / RNAプロセシング / 転写 / 組織特異性 / RNAseq / 遺伝子発現
Outline of Final Research Achievements

We developed new methods to label and purify nascent precursor messenger RNAs (pre-mRNAs) in a nematode worm C. elegans. With the methods, we revealed dynamics of pre-mRNA processing such as trans-splicing, cis-splicing and cleavage at a polyadenylation site for the first time in multicellular organisms. We also analyzed nascent pre-mRNAs in a splicing factor mutant and revealed effects of the factor on the dynamics of pre-mRNA processing.

Academic Significance and Societal Importance of the Research Achievements

mRNA前駆体のプロセシング動態が多細胞生物の個体レベルで明らかになるのは初めてである。哺乳類ではイントロンが長大であることから、同様の方法を適用しても同様の結果を得ることは実質的に困難である。本研究成果は、哺乳類を含む他の多細胞動物における転写と共役したmRNAの転写後プロセシングによる遺伝子発現制御機構を考察する上で基礎となる重要な知見を与えるものである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (34 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Book (1 results) Remarks (5 results)

  • [Int'l Joint Research] Emory University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Pittsburgh(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of California at Los Angeles/Emory University/University of Pittsburgh(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Institut Europien de Chimie & Biologie(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Emory University(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of California, Los Angeles(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Indiana University School of Medicine(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] mRNA Editing, Processing, and Quality Control in Caenorhabditis elegans2020

    • Author(s)
      Joshua A. Arribere, Hidehito Kuroyanagi, and Heather A. Hundley
    • Journal Title

      Genetics

      Volume: 215 Issue: 3 Pages: 1-39

    • DOI

      10.1534/genetics.119.301807

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner2018

    • Author(s)
      Watabe Eichi、Ono Shoichiro、Kuroyanagi Hidehito
    • Journal Title

      Cytoskeleton

      Volume: 75 Issue: 10 Pages: 427-436

    • DOI

      10.1002/cm.21489

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization2018

    • Author(s)
      Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura & Hidehito Kuroyanagi
    • Journal Title

      Scientific Reports

      Volume: 印刷中 Issue: 1 Pages: 8970-8983

    • DOI

      10.1038/s41598-018-26624-w

    • NAID

      120007031665

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans2018

    • Author(s)
      Barnes Dawn E.、Watabe Eichi、Ono Kanako、Kwak Euiyoung、Kuroyanagi Hidehito、Ono Shoichiro
    • Journal Title

      Molecular Biology of the Cell

      Volume: 29 Issue: 9 Pages: 1075-1088

    • DOI

      10.1091/mbc.e17-03-0152

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Alternative Splicing Regulator RBM20 and Cardiomyopathy2018

    • Author(s)
      Watanabe Takeshi、Kimura Akinori、Kuroyanagi Hidehito
    • Journal Title

      Frontiers in Molecular Biosciences

      Volume: 5 Pages: 105-105

    • DOI

      10.3389/fmolb.2018.00105

    • NAID

      120007150215

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization2018

    • Author(s)
      Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura and Hidehito Kuroyanagi
    • Journal Title

      Scientific Reports

      Volume: 印刷中

    • NAID

      120007031665

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An emerging model organism Caenorhabditis elegans for alternative pre-mRNA processing in vivo2017

    • Author(s)
      Shotaro Wani & Hidehito Kuroyanagi
    • Journal Title

      Wiley Interdisciplinary Reviews: RNA

      Volume: 印刷中 Issue: 6

    • DOI

      10.1002/wrna.1428

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Genome-Wide Kinetic Analysis of pre-mRNA Processing in C. elegans2019

    • Author(s)
      Eichi Watabe and Hidehito Kuroyanagi
    • Organizer
      The 24th Annual Meeting of the RNA Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genome-Wide Kinetic Analysis of pre-mRNA Processing in C. elegans2019

    • Author(s)
      Eichi Watabe and Hidehito Kuroyanagi
    • Organizer
      The 22nd International C. elegans Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PES-4 regulates head-muscle-specific alternative splicing of the tropomyosin pre-mRNA2019

    • Author(s)
      Eichi Watabe and Hidehito Kuroyanagi
    • Organizer
      The 22nd International C. elegans Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Homeostasis of S-adenosylmethionine (SAM) synthetase genes in C. elegans by alternative pre-mRNA splicing through m6A modification at the 3' spice site2019

    • Author(s)
      Shotaro Wani, Eichi Watabe, Yuma Ishigami, Marina Togo-Ohno, Tsutomu Suzuki and Hidehito Kuroyanagi
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Genome-Wide Kinetic Analysis of pre-mRNA Processing Reveals Action of Splicing Regulators2019

    • Author(s)
      Hidehito Kuroyanagi
    • Organizer
      Polish-Japanese RNA Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] トロポミオシンをコードするlev-11遺伝子の選択的スプライシング制御2019

    • Author(s)
      黒柳秀人
    • Organizer
      東京地区線虫勉強会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 動物個体におけるmRNA前駆体プロセシング動態の解析2018

    • Author(s)
      渡部栄地,黒柳秀人
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Genome-wide Kinetic Analysis of Pre-mRNA Processing in Living Animals2018

    • Author(s)
      渡部栄地,黒柳秀人
    • Organizer
      線虫研究の未来を創る会
    • Related Report
      2018 Annual Research Report
  • [Presentation] C. elegans代謝関連遺伝子のスプライシング制御における食餌の影響2018

    • Author(s)
      和仁翔太郎
    • Organizer
      東京地区線虫勉強会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genome-Wide Kinetic Analysis of Pre-mRNA Processing in Living Animals2018

    • Author(s)
      渡部栄地
    • Organizer
      東京地区線虫勉強会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 個体レベルで行うmRNA前駆体プロセシングの動態の解析2017

    • Author(s)
      渡部栄地、黒柳秀人
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] C. elegans代謝関連遺伝子のスプライシング制御における食餌の影響2017

    • Author(s)
      和仁翔太郎、黒柳秀人
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genome-wide kinetic analysis of pre-mRNA processing in living animals2017

    • Author(s)
      Eichi Watabe, Hidehito Kuroyanagi
    • Organizer
      CSHL Meeting on 40 YEARS OF mRNA SPLICING: FROM DISCOVERY TO THERAPEUTICS
    • Related Report
      2017 Annual Research Report
  • [Presentation] 個体レベルで行う mRNA前駆体プロセシングの動態の解析2017

    • Author(s)
      渡部栄地、黒柳秀人
    • Organizer
      ConBio2017(2017年度生命科学系学会合同年次大会)
    • Related Report
      2017 Annual Research Report
  • [Book] 遺伝子発現制御機構2017

    • Author(s)
      田村隆明、浦聖惠(編)
    • Total Pages
      264
    • Publisher
      東京化学同人
    • ISBN
      9784807909179
    • Related Report
      2017 Annual Research Report
  • [Remarks] 東京医科歯科大学 難治疾患研究所 フロンティア研究室 遺伝子発現制御学

    • Related Report
      2019 Annual Research Report
  • [Remarks] 東京医科歯科大学難治疾患研究所フロンティア研究室遺伝子発現制御学

    • Related Report
      2018 Annual Research Report
  • [Remarks] フロンティア研究室 遺伝子発現制御学

    • URL

      http://www.tmd.ac.jp/end/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 「ノンコーディングRNAネオタクソノミ」研究成果紹介

    • URL

      https://ncrna.jp/blog/item/370-rouge

    • Related Report
      2017 Annual Research Report
  • [Remarks] みらいぶプラス

    • URL

      https://www.milive-plus.net/newleader/049/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-05-23  

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