| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2017: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
V-ATPases function as ATP-dependent ion pumps. The hydrophilic V1 portion is known as a rotary motor in which a central axis DF complex rotates inside a hexagonally arranged catalytic A3B3 complex using ATP hydrolysis energy. We previously succeeded in obtaining the crystal structures of the A3B3 complex and the dynamics showing a unidirectional rotation of the complex in the presence of ATP analog by high-speed AFM. In this study, we elucidated the crystal structures and biochemical properties of ADP bound and ATP bound A3B3 complex. Based on these and previous findings, we propose a molecular mechanism of the unidirectional rotation of the rotor-less V1 rotary motor.
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