Molecular mechanism of the unidirectional rotation of the rotor-less V1 rotary motor
| Project/Area Number |
17H03638
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Chiba University |
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Principal Investigator |
Murata Takeshi 千葉大学, 大学院理学研究院, 教授 (80415322)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2017: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | V-ATPase / 分子モーター / 構造解析 |
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| Outline of Final Research Achievements |
V-ATPases function as ATP-dependent ion pumps. The hydrophilic V1 portion is known as a rotary motor in which a central axis DF complex rotates inside a hexagonally arranged catalytic A3B3 complex using ATP hydrolysis energy. We previously succeeded in obtaining the crystal structures of the A3B3 complex and the dynamics showing a unidirectional rotation of the complex in the presence of ATP analog by high-speed AFM. In this study, we elucidated the crystal structures and biochemical properties of ADP bound and ATP bound A3B3 complex. Based on these and previous findings, we propose a molecular mechanism of the unidirectional rotation of the rotor-less V1 rotary motor.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究よるA3B3複合体の分子メカニズムの解明は、V1モーターの協同的な反応機構の根本的な理解に重要であるばかりでなく、その他の分子モーターがもつ分子機構の一般原理の理解にも重要であると考えられる。また、V-ATPaseが関連するガン転移や骨粗鬆症などの重要な疾病原因の理解やその治療法についても進展することが期待される。
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Metastable asymmetrical structure of shaftless V1 motor2019
Author(s)
S. Maruyama, K. Suzuki, M. Imamura, H. Sasaki, H. Matsunami, K. Mizutani, Y. Saito, F. L. Imai, Y. Ishizuka-Katsura, T. Kimura-Someya, M. Shirouzu, T. Uchihashi, T. Ando, I. Yamato, T. Murata
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Journal Title
Sci. Adv.
Volume: 5
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] The combination of high-speed atomic force microscopy and X-ray crystallography reveals rotary catalysis of a shaftless V1 motor2019
Author(s)
Shintaro Maruyama, Motonori Imamura, Takayuki Uchihashi, Kazuya Nakamoto, Mizutani Kenji, Lica Fabiana Imai, Kano Suzuki, Yoshiko Ishizuka-Katsura, Tomomi Someya-Kimura, Mikako Shirouzu, Ichiro Yamato, Toshio Ando, Takeshi Murata
Organizer
第57回日本生物物理学会年会
Related Report
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[Presentation] The off-axis rotor of Enterococcus hirae V-type ATPase by Volta phase contrast cryo-EM2019
Author(s)
Burton-Smith Raymond. N, Tsunoda Jun, Yamamori Yu, Miyazaki Naoyuki, Imai Fabiana L., Song Chihong, Tomii Kentaro, Iwasaki Kenji, Takagi Junichi, Ueno Hiroshi, Murata Takeshi, Iino Ryota, Murata Kazuyoshi
Organizer
第57回日本生物物理学会年会
Related Report
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